| ObjectiveTo investigate the levels of vitamin B1,vitamin B2,vitamin B6,folic acid,vitamin B12 and vitamin C in serum of children with acute diarrhea,so as to explore whether there is abnormal levels of water-soluble vitamins in children with acute diarrhea,and to provide new ideas for the treatment of diarrheal disease.MethodsBeing the observation groups,a total of 73 children with acute diarrheal diseases who were hospitalized in Shenyang Children’s Hospital from October 1,2019 to February 28,2022 were collected which including 26 cases of viral enteritis group,31 cases of non-infectious diarrhea group and 16 cases of bacterial enteritis group.A total of 30 children with health check-ups were collected as the control group.All aged between 6 months and 6 years old.5ml fasting venous blood samples were collected after admission and before special treatment.Vitamins B1 and B2 were detected by high performance liquid chromatograph.The instrument model is Shimadzu lc-20a;Vitamin B6 and vitamin C are detected by liquid phase tandem mass spectrometry analyzer,and the instrument model is Agilent lc-ms-1260 / 6407;Vitamin B9（folic acid）and B12 were detected by chemiluminescence kit in automatic biochemical instrument maglumi4000 plus.Compare the severity of diarrhea,including the course of disease and the frequency of diarrhea,so as to comfirm the impact on water-soluble vitamins.The obtained data were analyzed and processed by SPSS statistics 23.0 software,the measurement data that conformed to the normal distribution were described by the mean ± standard deviation（?X±S）,while the non-normal distribution was described by the median and interquartile range [M（P25,P75）] description.Comparisons between two groups were performed using t（t’）test or nonparametric test（Mann-Whitney U test）,and comparisons between multiple groups were performed using analysis of variance（F test）or Kruskal-Wallis H test.Count data were recorded by frequency and percentage.The χ2 test was used for comparison among multiple groups.The multivariate analysis was performed by binomial logistic regression analysis.P<0.05 was considered statistically significant.Results1.There were 73 patients in the diarrhea group（45 males and 28 females）,including 26 viral enteritis patients（13 males and 13 females）,with an average age of（35.83±15.59）months;31 cases in the non-infectious diarrhea group（21 males and 10females）,with an average age of（40.97 ± 18.87）months;16 cases in the bacterial enteritis group,（11 males and 5 females）,the average age was（31.25 ± 14.47）months;as well as 30 patients in the control group（15 males and 15 females）,with an average age of（37.58±20.43）months.There was no statistical significance in gender and age composition（P>0.05）.2.There were 26 cases in the viral enteritis group,accounting for 35.6% of the diarrhea group,31 cases in the non-infectious diarrhea group,accounting for 42.5%,and 16 cases in the bacterial enteritis group,accounting for 21.9%.There was statistically significant difference in the results of metabolic acidosis in each subgroup of diarrhea（P<0.05）.There was no significant difference between the results of hypokalemia,hyponatremia,course of diarrhea,and frequency of diarrhea（P>0.05）.3.Comparing the results of water-soluble vitamins in the acute diarrhea group and the control group,the serum levels of vitamin B1,B6,and vitamin C in the diarrhea group were lower than those in the control group,and the difference was statistically significant（P<0.05）.But there was no significant difference about vitamin B2,folic acid and vitamin B12（P>0.05）.4.Compared the results of water-soluble vitamins among viral enteritis group,non-infectious diarrhea group,bacterial enteritis group and the control group indicated that the level of vitamin B1（Vit B1）in the non-infectious diarrhea group was lower than those in the bacterial enteritis group and the control group,the Vit B1 level in the viral enteritis group was lower than that in the control group,and the difference was statistically significant（P<0.05）.5.The 73 cases of children with acute diarrhea were grouped according to the course of the disease and the frequency of diarrhea,among which 51 cases had a disease duration > 3 days,and 22 cases had a disease duration ≤ 3 days.The levels of Vit B1,Vit B2,Vit B6,folic acid（FA）,Vit B12 and Vit C were compared according to the duration of the disease,the difference was not statistically significant（P>0.05）;there were 54 cases with diarrhea frequency ≤10 times/days,and 19 cases with diarrhea frequency >10 times/days.The result showed that Vit B1,Vit B2,Vit B6,FA,Vit B12 and Vit C levels had no significant difference（P>0.05）.6.Binary logistic regression analysis of serum vitamins B1,B6 and vitamin C,which may be related to the occurrence of diarrheal diseases in children,showed that vitamin B6（OR=0.911,P=0.002）and vitamin C（OR=0.408,P<0.001）were negatively correlated with acute diarrheal diseases.Serum vitamins B1 was not correlated with acute diarrheal disease（P>0.05）.Conclusion1.Acute diarrheal disease could lead to the reduction of water-soluble vitamin B1,B6,and vitamin C levels,and the most significant reduction in the non-infectious diarrhea group.2.The course of acute diarrhea and the frequency of diarrhea within one week had no significant effect on the levels of water-soluble vitamins.3.Serum vitamin B6 and vitamin C are closely related to children with acute diarrhea. |
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