Gene Identification And Signal Pathway Analysis Of Muscle Atrophy Induced By Excessive Exercise

Research Objective: Taking zebrafish as the research object,this study conducted intervention through excessive exercise to analyze the role of the signal pathway in promoting muscle atrophy of zebrafish skeletal muscle and verify the related factors of the signal pathway,so as to provide a reference for scientific fitness and in-depth research on excessive exercise.。Methods: Forty healthy male wild-type(WT)zebrafish were randomly divided into two groups: quiet control group(n=20)and overexercise group(n=20).80% Ucrit,100% Ucrit and 120% Ucrit were used for screening exercise intensity.The overexercise group was determined to exercise at 80% Ucrit swimming speed.Training seven days a week for eight hours a day.After exercise intervention,the skeletal muscle morphological characteristics of the overexercise group and the quiet control group were observed by weighing and HE staining.Marker genes for muscular atrophy were detected by q PCR.Measuring the limit swimming speed Ucrit observed the change of swimming ability;Measure the maximum oxygen uptake MO2 to judge the change of the maximum oxygen uptake;Second generation gene sequencing was performed to detect the signal pathways related to muscular atrophy and to verify the related signal pathway markers。Results: After the intervention of excessive exercise mode,body size and body weight of the overexercise group were significantly different from those of the quiet control group(P<0.01).He staining showed that the muscle cells in the quiet control group were plump and orderly,and the muscle nuclei were larger.In the overexercise group,the space between the muscle cells was larger,the cell arrangement was disordered,the nuclear area was smaller,and the muscle cells were atrophied to different degrees.Subsequently,the expression levels of muscular atrophy markers FBXO32,GADD45 BB and TRIM63 a in the overexercise group were significantly increased by validation of muscular atrophy marker factors(P<0.05),indicating muscular atrophy in skeletal muscle.Then,the ultimate swimming speed Ucrit and the maximum oxygen uptake MO2 were measured,and it was found that the Ucrit of the overexercise group was significantly decreased(P<0.05).MO2 was significantly decreased(P<0.01),and second-generation sequencing showed the autophagy,apoptosis,P53 signaling pathway,Forkhead box O(FOXO)signaling pathways,and P13K-Akt signaling pathways.Conclusion: 1.Overexercise training can establish the model of skeletal muscle atrophy in zebrafish 2.Excessive exercise is detrimental to performance and risks skeletal muscle loss 3.Overexercise induced muscular atrophy signaling pathways are associated with autophagy,apoptosis,P53 signaling pathway,Forkhead box O(FOXO)signaling pathways,P13K-Akt signaling pathways.