Metal-organic Frameworks (MOFs) Materials Are Used In The Fluorescence Analysis And Detection Of Biological Small Molecules

Metal organic frame usually refers to crystalline materials with periodic network structure formed by self-assembly of metal ions and organic ligands.MOFs can be designed reasonably,and their special adjustable properties have made them widely used in the past two decades in the fields of gas adsorption and separation,catalysis,chemical sensing,energy and drug loading.Aiming at the application of MOFs in the field of chemical and biological sensing,this paper studies the different functions and effects of three different types of MOFs as biomolecular probes.The structure,optical properties and surface properties of the samples were characterized and analyzed by means of SEM,XRD fluorescence spectrometer and FT-IR.Finally,the samples were used for fluorescence sensing analysis of small biological molecules.1.MIL-101(Cr)with strong adsorption was synthesized.Firstly,it was characterized by SEM,XRD,FT-IR,and its structure and optical properties were investigated.Then,the quenching effect of the synthesized metal-organic framework on FAM-labeled nucleotide sequence was studied.In this work,MIL-101(Cr)can effectively adsorb and quench adenosine triphosphate(ATP)and amplify the signal of nucleic acid exonuclease,which can make the detection limit of ATP lower than the previous level of analysis,confirming that the quenching substance has a good effect in biomolecular detection.Finally,a linear relationship between the fluorescence intensity of the fluorescent nanoprobe and the concentration of adenosine triphosphate was established,and the detection of adenosine triphosphate was successfully realized;2.A molecular fluorescence probe with selective recognition and high sensitivity was developed for the effective detection of glucose in vivo_In this chapter,the preparation of new nanocomposite Mn2+:ZnS QDs@ZIF-8@MnO2 and the detection of C6H12O6 are discussed.Firstly,a kind of Mn2+doped ZnS quantum dot(Mn2+:ZnS QDs)was synthesized to study its fluorescence properties;then,zeolite imidazole skeleton crystal ZIF-8 was synthesized at room temperature to realize the encapsulation of Mn2+doped ZnS quantum dots,and Mn2+:ZnS QDs@ZIF-8 composite was prepared to study its fluorescence properties;then,the composite was reacted with KMnO4 solution to prepare Mn2+:ZnS QDs@ZIF-8@MnO2 nanocomposite.Finally,the nanocomposites were reacted with different concentration gradients of glucose solution,and the fluorescence intensity of nanocomposites was measured to detect the concentration of glucose in organisms.3.Combining the porosities of MOFs with the special properties of nano-fluorescent materials,we designed and synthesized a kind of composite material Eu3+doped MIL-53(Fe)based on rare earth element doped metal organic skeleton,and emitted double fluorescence peaks in order to change the cholesterol content in the reaction system by changing the fluorescence signal ratio.Finally,the nanocomposites were reacted with different concentration gradients of cholesterol solution,and the linear relationship between cholesterol concentration and fluorescence ratio was determined by measuring the ratio of the two peaks of fluorescence intensity of nanocomposites,so as to achieve the purpose of detecting cholesterol concentration in organisms.