Effects Of Hydrogenated Soybean Oil On Glucose And Lipid Metabolism And Adipose Tissue Beiging In Mice

Hydrogenation of vegetable oils are the main source of Trans Fatty Acid(TFA).Ingestion of trans-fatty acids from food processing sources may significantly increase the risk of diseases such as obesity and diabetes,but its mechanism is not clear.There are beige adipocytes in white adipose tissue,which can participate in metabolic balance and body weight regulation.Therefore,hydrogenated soybean oil was added into the diets,and pressed soybean oil was served as control to analyze the effects of body weight and glucolipid metabolism,and analyzed the key to fatty acids composition,morphology and adipose tissue beigeing protein expression in mice.In vitro models of adipose stem cells were also used to compare the oleic acid with oleic acid and linoleic acid on adipogenic differentiation of adipose stem cells.In this experiment,mice were used as animal models.The mice were randomly divided into three groups:the soybean oil group(control group),and 1/4 hydrogenated soybean oil(1/4TFA group)or fully hydrogenated soybean oil(TFA group).The mice were fed freely for 9weeks,and body weight,abdominal fat ratio,liver index changes were observed.It was found that intake hydrogenated soybean oil significantly increased the body weight gain rate and abdominal fat ratio of the mice;meanwhile,the liver index was found to be significantly higher than that of the control group.Further analysis of serum biochemical and blood glucose level,found that the hydrogenated soybean oil intake could significantly increase TC content and decrease the content of HDL.TG and LDL had no significant.change It was suggested that the intake of hydrogenated soybean oil may cause abnormal lipid metabolism in mice.After the intake of hydrogenated soybean oil,there was no significant change in fasting blood glucose,but it could significantly reduce glucose tolerance.Analysis of fatty acid compositions in adipose tissue by GC-MS,showed that the intake of hydrogenated soybean oil significantly increased the content of trans-octadecenoic acid(T18:1)and elaidic acid in white adipose tissue,while the content of essential fatty acids?-linolenic acid and linoleic acid was significantly reduced.Histological staining and Western blotting were used to analyze the morphological changes of adipose tissue and the expression level of key protein of beige in adipose tissue.The results showed that hydrogenated soybean oil significantly increased the area of white adipocytes and decreased the expression level of uncoupling protein 1(UCP1)protein,but had no significant effect on the expression of cytochrome C.The results of analysis of brown adipose tissue showed that fatty acids compositions was significantly changed by hydrogenated soybean oil,which was in consistent with white adipose tissue,and the lipid droplets of brown adipocytes were increased.However,the expression of UCP1 did not change significantly.The results of differentiation of adipose stem cells in vitro showed that the addition of oleic acid or linoleic acid significantly promoted the formation of small lipid droplets in adipogenic differentiation of adipose-derived stem cells,while the oleic acid had no significant effect on the formation of small lipid droplets.In conclusion,it was found that the intake of hydrogenated soybean oil could increase the body weight and abdominal fat ratio of mice,induce abnormal metabolism of glucose and lipid,changed the fatty acids composition of adipose tissue,and significantly decrease the expression of UCP1 in white adipocytes.The results suggested that the effect of hydrogenated soybean oil may be related to the beige of adipose tissue.