Construction Of Deamylopectin-based-functional Active Substance Delivery System And Its In Vitro Controlled Release Effect

Debranching starch the starch has higher thermal stability and mechanical stability as food grade carrier due to its linear helix structure.The fine structure of debranching starch(DBLS)can affect the retrogradation mode,thus affecting its potential use as a delivery carrier of bioactive substances.In this study,high pressure,pullulanase debranching and/or ultrasonic cascade were used to prepare debranched lentil starch,and DBLS was used as the wall material to embed rutin to improve the solubility and stability of Rutin in water.Their encapsulation efficiency,structural characteristics,thermal stability,morphological features,antioxidant activity and in vitro release behavior under simulated upper gastrointestinal tract environment were evaluated.The results suggested that rutin was dispersed in the DBLS polymer matrix.The structural analyses of microparticles revealed that rutin could interacted with DBLS biopolymer chains by hydrogen bonds,making the starch molecular chains less susceptible to interact with themselves for reordering.The encapsulation efficiency was found to be in an opposite trend with those values obtained for relative crystallinity,melting enthalpy,degree of order/double helices of DBLS wall materials before encapsulation.The release rate results indicated that DBLS carrier with lower Mw,DPn and higher molecular order was beneficial for the slower release of rutin encapsulated in the microparticles.In addition,the cascade polysaccharide delivery system has better biocompatibility,biodegradability and higher modifiability,which can significantly improve the bioavailability of functional active ingredients.Thus,we prepared short chain debranched starch-?-carotene self-assembled delivery system,and explored the influence of multi-scale structure of short chain debranched starch on the delivery effect of ?-carotene by investigating the embedding efficiency of ?-carotene in different delivery systems,in vitro release behavior and ABTS inhibition capacity.It was found that the embedding efficiency of self-assembled particles for ?-carotene could reach 84.68-92.73%.The release of ?-carotene was triggered by the hydrolysis of short chain debranching starch by amylase,which was a controlled release process of enzyme and pH.?-CC-S24 was the most ideal self-assembly embedded particles,which can not only minimize the release of ?-carotene in the stomach,prevent the isomerization of ?carotene in acidic conditions,and generate oxygen promoting compounds that are not conducive to human health,but also keep a uniform and slow release at the absorption site(small intestine)of ?-carotene,avoiding the accumulation of ?-carotene caused by sudden or rapid release,which was conducive to improving the bioavailability of ?carotene,promote the transformation of ?-carotene into vitamin A needed by human body,and give a play to physiological benefits.