Study On Preparation And Quality Control Of The Reference Substance Of Taizishen

In this paper,heterophylla as the main object,as heterophylla Preparation of reference,quality control heterophylla medicine research.First Taizishen polysaccharides were isolated and purified using phenol-sulfuric acid method for our laboratory to determine the content Taizishen refined polysaccharide in an amount more than 80%.Made use of the laboratory heterophylla heterophylla purified polysaccharide as the reference medicine,phenol-heterophylla medicine in the polysaccharide content determined by the method were investigated sulfuric acid method,the results showed that the precision,repeatability,stability and sample recovery rates to meet the requirements,determined the content of polysaccharides in eight lots heterophylla medicinal use of this method.In this study,Taizishen polysaccharides monosaccharides were identifiedby TLC and pre-column derivatization method-HPLC,these two methods are required to first polysaccharide hydrolysis,high temperature used in this experiment is a strong acid method.Polysaccharide hydrolyzate obtained first by TLC a preliminary identification method to determine the TLC eluent: ethyl acetate: pyridine: ethanol: water = 8: 1: 1: 2;reagent: 2%aniline: diphenylamine 2%: 85% phosphoric acid = 5: 5: 1;color temperature should not exceed 100 ?: the color of the time within 5 min.Combining color development and ratio shift value,wherein the determining comprises rhamnose and xylose,glucose and galactose may contain,fructose does not exist.Then the front-column derivatization method-HPLC Taizishen polysaccharide monosaccharide composition further identification of the hydrolyzate with 1-phenyl-3-methyl-5-pyrazolone(PMP)derivatized reaction,derivatized product was purified by HPLC detection can be determined heterophylla polysaccharide contains rhamnose,glucose,galactose and xylose monosaccharide according to the retention time of each component.In this study,rhamnose,glucose,galactose and xylose monosaccharide four kinds of reference for heterophylla medicine in a single sugar content were studied.Firstly,the single factor method for liquid chromatographic conditions were screened,under optimum conditions hydrolysis conditions on the chromatographic conditions of polysaccharides andderivatives reaction screening,ultimately determine the method heterophylla medicine in the determination of the monosaccharide,and this method is a method validation,precision,repeatability,stability meet the requirements,the use of this method for eight lots in a variety of single herbs heterophylla sugar content were determined.