Comparative Analysis Of The Use Of Violin Repair Fixture

Polyphenols has a profound effect on skin whitening and beautifying.They are the main ingredients in beautifying products.Kiwifruit peel is rich in polyphenols and it can be used as raw materials for beautifying and health care products.This paper aimed to study the extraction and purification of polyphenols from kiwifruit peel,the mechanism of whitening and beautifying,and the preparation of whitening mask solution.In this paper,extract polyphenols from kiwifruit peel with deep eutectic solvents（DESs）,and the process parameters were optimized.The recovery method was adopted to separate and enrich the polyphenols of kiwifruit peel.The polyphenols extracted of kiwifruit peel after separation and enrichment was determined by HPLC,and the antioxidant activity and tyrosinase inhibition of polyphenols in kiwifruit peel were studied in vitro.The mouse B16 cells induced by the inducerα-MSH were used as the research object,to investigate the inhibitory result of kiwifruit peel polyphenols on B16 cells on tyrosinase activity and melanin production.A whitening facial mask liquid was prepared by using the extract of kiwifruit peel polyphenols,and the physicochemical properties.And measurement of antioxidant capacity and tyrosinase activity of facial mask solution.The results of the study were as follows:1.Acquisition of kiwifruit peel polyphenols and majorization of process parametersUsing kiwifruit peel as raw material,the effects of eight DESs on the yield of kiwifruit peel polyphenols were studied,and compared with water,methanol and ethanol solvents,the results showed that the use of DES-1（choline chloride and ethylene glycol were mixed in a molar ratio of 1:2）to abstract polyphenols was the best.Based on the factors of DES-1 water content,solid-liquid ratio,extraction time,extraction temperature,etc.,a response surface design experiment was carried out.The water volume of DES-1was 18%,and the ratio of solvent to DES-1 solution was 1:29 g/m L,time was 22 min and temperature was 69℃,at that time yield of kiwifruit peel polyphenols was the highest,which was（55.53±1.27）mg/g.The obtained kiwifruit peel polyphenols extract is abbreviated as KPP crude extract.2.Enrichment and composition survey and evaluate of polyphenols in kiwifruit peelBy exploring the influences of resin type,adsorption time,and desorption time,dilution ratio of KPP extraction solution and desorption solution concentration on the separation and enrichment of KPP extracted by DES-1.The experiment showed that the KPP extraction solution was diluted 10 times and adsorbed with X-5 macroporous resin for 60 min,when 60%ethanol was desorbed for 120 min,the separation and enrichment effect was the best,and the recovery was（95.42±2.48）%.The composition of the KPP extract after separation and enrichment was determined by HPLC,and a total of 10polyphenol monomers were detected,including（+）-catechin,chlorogenic acid,caffeic acid and epicatechin.3.Antioxidant capacity of polyphenols in kiwifruit peel and its effect on tyrosinaseThe antioxidant capacity of KPP and its suppression function on tyrosinase activity were determined.The outcomes revealed that KPP had good antioxidant capacity.The scavenging ability of DPPH free radical was as follows:KPP(IC₅₀=20.93μg/m L)>ascorbic acid(IC₅₀=27.56μg/m L).The scavenging ability of ABTS free radical was as follows:KPP(IC₅₀=41.91μg/m L)>ascorbic acid(IC₅₀=46.73μg/m L).The reduction ability of FRAP was ascorbic acid(IC₅₀=84.66μg/m L)>KPP(IC₅₀=116.83μg/m L).The KPP had a good inhibitory effect on TYR,and the inhibition rate was 74.45%at 2.6mg/m L.4.Researched on the whitening effect of kiwifruit peel polyphenols based on B16 cellsKPP had no influence on the relative cell viability of B16 cells and had no toxic effect.The cell morphology did not variety remarkably when B16 cells were treated with KPP.KPP had inhibitory effects onα-MSH-induced intracellular melanin content and tyrosinase activity in B16 cells,and the inhibitory function is more powerful than that of kojic acid.After treatment with KPP the activity of tyrosinase in B16 cells declined to（69.45±6.82）%,the content of melanin in B16 cells declined to（75.08±1.64）%,and the whitening effect was obvious.5.Preparation and index determination of kiwifruit peel polyphenols whitening facial mask liquidThe mask solution prepared from the KPP is uniform and bright,which was in line with the industry standard,and had a certain antioxidant activity.The inhibition rate of tyrosinase activity reached 50.06%,showed that the kiwifruit peel polyphenols mask solution had whitening effect on biochemical level.