Immobilization Of Phenol-degrading Bacteria And Their Purification Study Of Coal Gasification Wastewater

Underground coal gasification（UCG）is the controlled in-situ combustion of coal stored underground to produce coal gas,which is of great interest to major coal-producing countries because of its significant economic and environmental benefits,and is known as"green mining".However,the pollution of groundwater by UCG is one of the major environmental risks,which may cause organic and inorganic pollution of groundwater.In this paper,JHFS-1,a microbial strain that can effectively degrade coal gasification wastewater,was cultivated and domesticated from coking wastewater and gasification wastewater,and the optimal degradation conditions of phenol,and the effects of Na Cl,Cu²⁺and Mn²⁺on the degradation of phenol by JHFS-1 were investigated with coal-based activated carbon.treatment,the degradation of organic pollutants in simulated gas scrubber water by JHFS-1 and immobilized JHFS-1 was investigated,and the following results were obtained.Using phenol as the only carbon source,three strains of phenol-degrading bacteria JHFS-1,JHFS-2 and QHFS-1 were isolated from coal gasification wastewater of a plant in Jincheng City and coking wastewater of a plant in Gongyi City,and JHFS-1 and QHFS-1 had good degradation effects on phenol.After growth characterization and 16S r DNA identification,the two are the same strain of Acinetobacter calcoaceticus sp.The effect of temperature,p H,shaker speed,inoculum and Na Cl concentration on the degradation of phenol by JHFS-1 showed that the best conditions for phenol degradation by JHFS-1 strain were:30℃,p H 6.0,shaker speed 120 r/min,inoculum13%,and the degradation rate of phenol solution of 500mg/L reached 94.31%after 24h.2%Na Cl had a significant inhibitory effect on the degradation of phenol by JHFS-1.The high content of Cu²⁺and Mn²⁺in coal gasification wastewater was selected to study the effect of heavy metals on the degradation of phenol.Cu²⁺had basically no effect on the degradation of phenol by JHFS-1 at low concentrations,but the effect was obvious at higher Cu²⁺concentrations;Mn²⁺had no effect on the degradation of phenol by JHFS-1;kinetic studies showed that the growth kinetics of JHFS-1 strain was in accordance with the Haldane equation,in which the half-saturation coefficient K_s=470.289 mg/L,the substrate inhibition coefficient K_i=664.201 mg/L.The degradation of phenol by JHFS-1 was presumed to follow the hydroxylation pathway by GC-MS and FT-IR analysis.The microbial immobilization technique was used to immobilize JHFS-1,and the immobilized JHFS-1 bacteria showed improved tolerance to phenol concentration,p H and Na Cl,and the degradation rate of phenol was still over 90%after 20 times of reuse.JHFS-1 bacteria were effective in degrading organic matter in gas scrubber water.The simulated degradation experiments showed that the degradation rate of gas washing water containing 278.83 mg/L TOC was 58.43%and 64.07%for native JHFS-1 bacteria and immobilized JHFS-1 bacteria,respectively,while the content of phenols,indoles and quinolines in gas washing water decreased significantly.The paper includes 46 figures,14 tables and cites 81 references.