Preparation Of Biocompatible Photoresponsive Surface Molecularly Imprinted Polymers And Delivery Of Anticancer Drug Molecules

Biocompatible photoresponsive surface molecularly imprinted polymers are bio-intelligent materials developed by combining biocompatible polymers with photoresponsive surface molecular imprinting technology.Compared with ordinary photoresponsive molecularly imprinted polymers,surface molecularly imprinted polymers effectively reduce the phenomenon of"embedding"of imprinted sites,have large binding capacity with template molecules,fast migration rate,and high recognition efficiency.In recent years,photoresponsive molecularly imprinted polymers have been used by a wide range of researchers for drug delivery due to their excellent recognition and light modifiable properties.However,most of the photoresponsive molecularly imprinted polymers are poorly biocompatible,limiting their application in the biological field.In this paper,biocompatible photoresponsive surface molecularly imprinted polymers were prepared for anti-cancer drug delivery by using biocompatible functional monomers and cross-linking agents.In this paper,a biocompatible photoresponsive functional monomer diazocine derivatives and a biodegradable cross-linkers dimethyl acryloyl hydroxylamine,were designed and synthesized.Two biocompatible photoresponsive surface molecularly imprinted polymers were prepared through bulk polymerization on the surfaces of silica and MOF material UiO-66,respectively.The effect of reaction conditions on the polymer preparation was investigated,the materials were characterised by SEM,FT-IR,and BET adsorption,the photoresponsive properties of the functional monomers and materials were investigated,and the adsorption and release properties of the materials were evaluated.This paper contains the following four chapters:1.In chapter one,an overview of the main advantages and disadvantages of molecularly imprinted polymers and surface molecularly imprinted polymers were summarized;the common substrate materials for surface molecularly imprinted polymers,preparation methods and applications were introduced.The poor biocompatibility of currently available surface molecularly imprinted polymers greatly limits their application in the biological field,and therefore the use of molecularly imprinted polymers for drug delivery faces a great challenge.In order to enhance the biocompatibility of the material,research advances in biocompatible photoresponsive units and crosslinkers are analysed.The aim and research significance of this thesis is to develop and prepare biocompatible photoresponsive surface molecularly imprinted polymers for the delivery of drug molecules.2.In chapter two,a visible light-responsive surface molecularly imprinted polymer was prepared for the delivery of the anticancer drug 5-fluorouracil（5-Fu）.The visible light-responsive surface molecularly imprinted polymers（SiO₂-MPS@VSMIP）was successfully prepared by bulk polymerisation with a visible light-responsive 3-（3’-aminodiazoicin）methacrylamide as the functional monomer,trimethoxypropyl trimethacrylate as the crosslinker,5-Fu as the template molecule and silica nanoparticles as the substrate.The preparation conditions,photoresponsive and adsorption properties of the materials were investigated,and the morphology and structure of the materials were characterised.The SiO₂-MPS@VSMIP loaded with 5-Fu was tested for in vitro release,which showed good slow release of the anti-cancer drug molecule 5-Fu.The results indicate that SiO₂-MPS@VSMIP is an excellent drug carrier material.3.In chapter three,a biodegradable photoresponsive surface molecularly imprinted polymer was prepared for the delivery of the anticancer drug molecule temozolomide（TMZ）.The biodegradable photo-responsive surface molecular imprinting polymer（DMHA@SMIP）was successfully prepared by bulk polymerisation using 4-[3,5-dimethyl-（4’-methacryloyloxy）azobenzene]benzene sulphonic acid as the functional monomer,biodegradable dimethyl acryloyl hydroxylamine（DMHA）as the cross-linker,TMZ as the template molecule and the shape-regular MOF material UiO-66 as the substrate.The photoresponsive and adsorption properties of the materials were investigated and their morphology and structure were characterised.The degradability of DMHA@SMIP materials was investigated in vitro by simulating physiological body fluids at different p H levels,which show significant biodegradation under neutral or weakly alkaline conditions.The DMHA@SMIP loaded with TMZ was tested for in vitro release and the material showed good slow release of the anti-cancer drug molecule TMZ,which demonstrates it’s an excellent drug carrier material.4.In chapter four,the research results of this paper are summarized.Future research work is prospected and directions for further optimizing material properties are proposed.