Construction Of Wearable Biofluids Sensors Based On Carbon Nanotubes For Non-invasive Monitoring

Compared with traditional centralized medicine,personalized medicine can realize the prevention,early diagnosis,and management of diseases,which is expected to change the medical status quo.Wearable biofluids sensors for personalized medicine are attracting attention due to the non-invasive monitoring of biochemical markers in biofluids such as sweat,tear,saliva,and urine,which can provide continuous and real-time physiological information.Wearable biofluids sensors can sample the biofluids in a non-invasive manner without damaging the surface of the skin and are therefore more user-friendly.Compared with other preparation methods,screen printing technology is easy to operate and does not require expensive instruments,and can be easily patterned into flexible electrodes,so it is widely used in the preparation of wearable biofluids sensors.In our work,two wearable biofluids sensing platforms were constructed.They can provide rich information about human health and realize real-time personalized monitoring of human health status.The specific contents are as follows:(1)The carbon nanotube-polysiloxane composites(CNT-putty)were successfully synthesized,and a wearable glove sensor was constructed on the basis of glove using screen printing technology.The composite has the advantages of simple composition,easy synthesis,and better electrochemical performance than commercial screen-printing electrode.The glove sensor does not need to be modified with other materials.The three-electrode system consisting of CNT-putty working electrode,Ag reference electrode and CNT-putty peer electrode is used for electrochemical detection of uric acid(UA)in biofluids,which has good linearity,selectivity and low detection limit.Integration of glove sensor with the portable electrochemical workstation,Palm Sens,enables the construction of sensing platform.Compared with traditional detection instruments,it has the advantages of easy portability,low cost,convenient use,and fast response.In order to evaluate the practical application possibility of the glove sensing platform,the real-time detection of UA in sweat,saliva and urine under different dietary conditions was performed by using the sensing platform.The relatively accurate results indicate that the glove sensing platform is expected to be used for personalized monitoring and management of UA levels.(2)Based on the performances of CNT-putty in wearable glove sensing platform,a wearable epidermal sensing platform based on CNT-putty was further designed and constructed in this study.The wearable epidermal sensing platform consists of a sensing module,a microfluidic module and a portable electrochemical workstation(Palm Sens),which can realize efficient microfluidic collection of human sweat and highly sensitive in-situ detection of UA,Na~+and lactic acid(LA)in sweat.The epidermal sensing platform is used to detect UA,Na~+and LA in sweat.The detection results are in good agreement with commercial kits and atomic absorption spectrometers(AAS),indicating that the sensing platform has good accuracy.In addition,we investigated the association of the concentration of UA,Na~+and LA in sweat with the level of human health,and evaluated the potential applications of the platform in diet management,gout risk reduction,dehydration identification,and health management.