| This paper prepared three categories of antibacterial agents:sulfamazine/sodium alginate/polyvinyl alcohol compound antibacterial gel ball pellet,silver sulfamazine(sulfamazine copper)/sodium carboxymethyl cellulose(sodium alginate)/polyvinyl alcohol compound hydrogel,different molecular weight of polyethylene glycol with sulfamazine(PEG_n-SD)sustained-release antibacterial sponge,and its properties were studied.The sulfamazine was first wrapped in the gel structure of sodium alginate by physical crosslinking by direct droplet addition,and the coated sulfamazine ion and the copper ion were released by dissolving the gel beads and water.Conclusion:The addition of 0.15g of sulfadiazine was used,drug loading and encapsulation rate were29.59%and 97.96%,respectively.After the in vitro release test,the cumulative release rate of the released drug was 26.97%in the first two hours,and there was no sudden release phenomenon,and it was gradually increased with the increasing p H.The minimum inhibitory concentration against S.aureus was at 15.62μg/m L,which almost achieved the sterilization effect at 250ug/m L,and the minimum inhibitory concentration for E.coli was 31.25μg/m L.In the water absorption rate in the deionized water is 204.65%,it is believed that the material has a super water absorption energy,can be used as a dressing for antibacterial and anti-inflammatory at the same time can play the function of suction fluid and hemostasis.Secondly,the positively charged silver sulfadiazine(SD-Ag)and sulfadiazine copper(SD-Cu),The products of the electrostatic adsorption were SA-SD-Ag,CMC-SD-Ag,SA-SD-Cu,and CMC-SD-Cu.Conclusion:The electrostatic adsorption of SD-Cu and SD-Ag by CMC and SA increased the dissolution of SD-Cu and SD-Ag,and the addition of PVA improved the water dispersion and hydrophilic energy of SD-Cu and SD-Ag.The minimum inhibitory concentrations of SA-SD-Ag against E.coli and S.aureus were 31.25μg/m L and 15.62μg/m L,respectively;the minimum inhibitory concentrations of CMC-SD-Ag against E.coli and S.aureus were 61.25μg/m L,respectively;SA-SD-Cu was 15.62μg/m L and 7.81μg/m L,respectively;and CMC-SD-Cu was 15.62μg/m L and 7.81μg/m L,respectively.The comparison found that compared with the SD-Ag drug,the SD-Cu inhibited E.coli and Staphylococcus aureus better,and the four antibacterial agents inhibited the Gram-positive bacteria better than the Gram-negative bacteria.The four antibacterial agents were added to the hydrogel prepared in PVA and tested in the antibacterial sphere.The transparent circle of E.coli was greater than or equal to 1cm,it is concluded that the matrix will not destroy the antibacterial properties of the antibacterial drug,and can be used as the matrix of the antibacterial agent.Finally,antibacterial agents of PEG2000-SD,PEG1000-SD and PEG600-SD with different molecular masses were prepared.Conclusion:As the relative molecular mass of polyvinyl alcohol decreases,its esterification degree also increases,and the amount of grafted SD will also increase.The minimum inhibitory concentrations of PEG2000-SD for E.coli and S.aureus were 125μg/m L and 125μg/m L,respectively,PEG1000-SD was 62.5μg/m L and 31.25μg/m L,and PEG600-SD was 31.25μg/m L and 15.62μg/m L,respectively.With decreasing relative molecular weight,the better.The inhibition diameter of E.coli and S.aureus was 4.0cm and 4.2cm by preparing a PEG600-SD/PVA hydrogel,respectively.Preparation into a PEG600-SD/PVA hydrogel does not aff-ect the antimicrobial properties of the antimicrobial agents and can be used as a matrix for antimicrobial agents. |
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