Preparation And Drug Release Properties Of Drug-loaded Nanofiber Membaranes Based On Emulsion Electrospinning

With the improvement of the medical level and the need for pathological research,loading multiple drugs and controlling the burst release and differential release of multiple drugs has become a current research hotspot.By combining macromolecular self-assembly technology and electrospinning technology,the hydrophilic drug model is wrapped in self-assembly colloidal particles,and the corn oil with colloidal particles as particle emulsifier with hydrophobic drug model is obtained by O/W type emulsion,and the drug-loaded nanofiber membrane is obtained by emulsion electrospinning.The specific research content is divided into the following parts:（1）Dual drug-loaded nanofibers membranes via emulsion electrospinning based on VB₁₂/Lys/CMC NPsIn this work,a spherical VB₁₂/Lys/CMC colloidal particle of approximately 334 nm was prepared from sodium carboxymethylcellulose（CMC）,Lysozyme（Lys）and hydrophilic drug(VB₁₂)under weak interaction by macromolecular self-assembly technique.Subsequently,an oil-in-water emulsion was obtained by using VB₁₂/Lys/CMC colloidal particles as emulsifiers,gelatin as spinning aid,and corn oil containing hydrophobic drug（VD₃）as oil phase.Dual drug-loaded core-shell nanofibers were further obtained by emulsion electrospinning.（2）Triple drug-loaded nanofibers membranes via emulsion electrospinning based on VB₁₂/Lys/CMC NPsAn oil-in-water emulsion was obtained by using VB₁₂/Lys/CMC colloidal particles as emulsifiers,gelatin as spinning aid,amlodipine has been added into the aqueous phase,and corn oil containing hydrophobic drug（VD₃）as oil phase.Triple drug-loaded core-shell nanofibers were further obtained by emulsion electrospinning.（3）Triple drug-loaded nanofibers membranes via emulsion electrospinning based on VB₁₂/HA/CS NPsIn this work,a spherical VB₁₂/HA/CS colloidal particle of approximately 300 nm was prepared from Chitosan（CS）,hyaluronic acid（HA）and hydrophilic drug(VB₁₂)under weak interaction by macromolecular self-assembly technique.Subsequently,an oil-in-water emulsion was obtained by using VB₁₂/HA/CS colloidal particles as emulsifiers,gelatin as spinning aid,amlodipine has been added into the aqueous phase,and corn oil containing hydrophobic drug（VD₃）as oil phase.Triple drug-loaded core-shell nanofibers were further obtained by emulsion electrospinning.The structure and morphology of drug-loaded nanofiber membranes were characterized by SEM,TEM,XRD and FTIR,and the cytotoxicity experiment was performed by CCK-8 method,which was proved that the prepared drug-loaded fiber membranes were non-toxic and had good biological activity.In vitro release experiments demonstrated that the drug-loaded nanofibers prepared by emulsion electrospinning showed slow release and differential release of VB_12,VD₃ and amlodipine.