Research And Development Of The Process Of Theabrownin Gel Candy And Its Lipid-lowering Effec

The production of theabrownins by liquid fermentation with Aspergillus niger was carried out using the remaining broken tea powder and low-quality tea as raw materials.Functional gel fudge-theabrownins gel fudge was developed by using the physiological activity of theabrownins to reduce blood lipid.Firstly,the chemical composition of liquid fermented theabrownins was analyzed.The optimal single factor parameter conditions of theabrownins soft candy were determined by single factor experiment.The production process parameters of theabrownins soft candy were further optimized by response surface test design to determine the optimal process parameters and verify them.The hypolipidemic activity of theabrownins soft candy in vitro was studied.The physicochemical properties and microbial indexes of theabrownins soft candy were determined to provide feasible data for its subsequent production.The specific research contents are as follows:(1)The chemical composition of theabrownins was analyzed and the process parameters of theabrownins soft candy were optimized.Compared with theabrownins produced by solid fermentation,the total phenols,amino acids and proteins in theabrownins produced by liquid fermentation were significantly different(P < 0.01).The total phenolic content of theabrownins produced by liquid fermentation was22.78 %,the amino acid content was 4.74 %,and the protein content was 22.47 %.The optimum proportion of each factor was determined by single factor experiment as follows: theabrownins addition amount 4 %,gel addition amount 8 %,xylitol addition amount 6 %,citric acid addition amount 0.1 %.The optimum process parameters of theabrownins gel soft candy were 4.69 % of theabrownins,6.33 % of xylitol,0.11 % of citric acid and 7.90 % of gel.The theoretical value of sensory evaluation under this condition was 86.67.The sensory evaluation score after optimization was 86.29 ± 0.15,and the error value was only 0.38.(2)To analyze the hypolipidemic activity of theabrownins soft candy.Through the determination of bile salt binding ability,when the concentration of liquid fermented theabrownins soft candy was 6 mg / m L,the inhibition rate of glycocholate was 44.12%,and the inhibition rate of taurocholate was 28.51%.The results of the inhibition of pancreatic lipase showed that when the sample concentration was 6 mg / m L,the inhibition rate of pancreatic lipase was 56.04 %.The determination results of cholesterol adsorption capacity showed that under acidic conditions,the cholesterol adsorption capacity of liquid fermented theabrownins soft candy was 93.43 mg / g.(3)Determination of physical and chemical and health indicators.The weight loss rate of theabrownins soft candy was 17.24 %.The total number of colonies in each gram of theabrownin gel soft candy was 63 CFU,and the coliform group was not detected.The above indicators met the limit values specified in the national standard.