Studies On The Composition And Digestive Characteristics Of Milk Fat Globular Membrane From Different Milk Sources

In order to meet the needs of infants who cannot be breast fed due to various conditions,research and exploration of alternatives to bioactive substances in breast milk have become an important element in the innovation and development of infant formula milk powder,of which MFGM,as one of the important ingredients in milk,is gradually gaining attention and is being used in infant formula milk powder.However,the composition,function and digestive properties of milk fat globules and their membranes in milk fat from different dairy sources(bovine,sheep and camel)are not fully understood.Therefore,in this study,MFGM lipids and proteins from different dairy sources(bovine,sheep and camel)were extracted and analysed separately for differential quantitative and compositional analysis.And by simulating infant gastrointestinal digestion and establishing in vitro models to explore the digestive characteristics and differences of MFG components of different milk sources,the aim is to continuously research and explore the differences in MFGM components of different milk sources and the digestive characteristics of MFG,providing a new direction for the upgrading and iteration of infant formulae,as well as providing new ideas for the development of non-bovine milk by-products.The specific results are as follows:(1)Comparative analysis of the lipid composition in MFGM from different milk sources.A total of 18 types of lipid subcompounds were identified in lipid globular membrane from different milk sources.It mainly includes three categories of Glycerolipids(GL),Glycerophospholipids(GP),and Sphingolipids(SP).The proportion of saturated glycerides in the fat globule membrane of cow milk was the highest;The proportion of saturated sphingomyelin in the fat globule membrane of goat milk was the highest;The proportion of total glycerides in the fat globule membrane of camel milk was the highest,and the content of phospholipids was higher than other milk sources.(2)Comparative analysis of membrane protein composition of milk fat globule from different milk sources.Compared with other milk sources,the number of upregulated protein of camel milk fat globular membrane protein was 309 and 315,respectively.Differential proteins are used for functional annotation.Differential proteins are mainly involved in cellular components,molecular functions and biological processes.KEGG pathway analysis showed that the upregulated differential proteins in the fat globule membrane of most camel milk were related to various physiological activities such as protein processing and material transport.(3)Simulated infantile in vitro digestion test.By static light scattering laser particle size analyzer,it was found that the size of milk fat pellets from different milk sources decreased with the digestion time.The absolute value of potential measured by dynamic light scattering potentiometer showed that all milk fat globules from milk source showed strong electronegesis after digestion in gastrointestinal tract.There was no significant difference in the free fatty acid release rate of milk fat pellets from different milk sources during intestinal digestion.