Design Of Intelligent Liquid Injection Device For In Situ Culture Of Microorganisms In Deep Sea Sediments

The deep sea accounts for 75% of the total ocean volume of the earth,and it is a precious and unknown land with great development potential.With the continuous exploration of scientists,the mystery of the deep sea ecosystem was gradually revealed after the discovery of independent life systems in the deep sea.The deep-sea ecosystem is dominated by microorganisms.Due to their special living environment,these extremophiles have unique survival mechanisms and characteristics.The deep-sea sediments are mainly formed by the sedimentation and accumulation of upper seawater particles,covering almost the entire seabed,and containing huge microorganisms.Therefore,it has a strong scientific research value.Single-factor control experiments are one of the important methods of life science research.However,conventional laboratory simulation culture and deep-sea sampling research have certain limitations.Therefore,the development of in-situ microbial culture and monitoring equipment is an important way to support deep-sea life science research best means.This paper summarizes the existing deep-sea in-situ devices,and based on the practical application point of view,designs and implements an intelligent liquid injection device that can be installed in the long-term culture system of deep-sea sediment microorganisms.The thesis will conduct detailed research on the device in the following aspects.First,the background and significance of the research and development of the intelligent liquid injection device are given,and the importance of in situ single-factor induction culture is summarized.Secondly,according to the actual application requirements,the design indicators and goals of the intelligent liquid injection device were formulated,and the overall scheme design was carried out.The device was divided into a controllable slow-release liquid injection system and a pore water collection and monitoring system for detailed analysis.Then,according to the overall plan,a detailed design study was carried out on the deep-sea low-speed liquid injection motor,large-capacity liquid storage tank,deep-sea micro peristaltic pump,pore water replacement collection cabin and other components,and the calculation and verification of key components were carried out,and ABAQUS software was used The static simulation verification is carried out,and the flow field analysis of the pore water displacement collection process is carried out by using FLUENT software,which provides a basis for the design of the pore water collection chamber and the sensor monitoring strategy.After completing the driving test,displacement test and pressure test of the core components,the device was integrated into the deep-sea sediment insitu experimental system,the software and hardware control modules were designed and debugged,and then the whole machine test was carried out.The test and three sea trials have found existing problems and verified the rationality of the device design,which has strong application value.Finally,the overall work of the paper is summarized and the future work is prospected.