Transcriptome Analysis Of Paphia Textile With Different Foot Colors

Paphia textile is one of the most economically important bivalve species in marine organism.The study on the distribution of total carotenoids(TCC)in different tissues of P.textile and the molecular mechanism of foot color difference provide higher reference for breeding P.textile with high economic value in the next step.In this study,we analyzed the distribution of TCC in different tissues of P.textile.Then,the foot transcriptome of P.textile with different color were analyzed using the second and third-generation sequencing technology.The research results were as follows:(1)One hundred P.textile individuals were selected to compare TCC in five tissues including foot,mantle,adductor muscle,gill and gonad.The results showed that TCC in five tissues from high to low are as follows:foot,gonad,gill,mantle,and adductor muscle.There were extremely significant differences among five tissues(P<0.01)except the gill and gonad for TCC.There was no significant difference in foot,mantle,gill,adductor muscle for TCC between male and female P.textile,but the TCC in testis was higher than ovary(P<0.01).(2)The foot transcriptome of P.textile with different color were analyzed by the second and third-generation sequencing technology.Analysis of gene differential expression was also performed in the orange and the white feet of P.textile.The second-generation sequencing results showed that the clean data of each sample reached 6.90Gb,the GC content was 35.30%～38.21%,and the percentage of Q30 base was 94.43%or above.The results of third-generation sequencing showed that after the correction of the low-quality full-length transcripts,the orange foot group and white group obtained 16,968 and 21,611 high quality sequences,respectively.Among them,high quality transcript sequences were compared with the major databases,5058 full-length transcripts with annotated information were obtained.Using RSEM software to statistical the comparison results of Bowtie 2,a total of 57 differentially expressed genes(DEGs)were obtained,including 34 up-regulated and 23 down-regulated genes in orange group.These DEGs were involved in many intracellular signaling pathways such as PPAR,FoxO,PI3K-Akt signaling pathway and Glycolysis,which formed a potential regulatory network that affects the color of foot.The results of this experiment pave the way for further exploring the molecular mechanism of color-related genes and assist selective breeding practices in high economic value P.textile.