| Wheat is an important food source for human beings.Disease resistance and high yield have always been the goal of breeding.Due to the self-regulation of crops in the face of external conditions,it is often difficult to achieve the maximum advantage at the same time.Especially,through the existing crop empirical breeding methods to obtain high-quality and high-yield agronomic characters and ideal disease resistance seem to be an idealized point of view.Conversely,crop breeders must balance immunity,resistance and high yield traits when selecting breeding populations.Unfortunately,the basic research on yield-resistance correlation is still in a blank stage due to the lack of yield level testing conditions under sterile conditions and the lack of varieties with the same background but different in resistance/susceptibility.Here,we focused on wheat yield(grain size)and disease resistance respectively to explore the crosstalk relationship between wheat growth and immunity from two aspects of wheat yield(grain size)and disease resistance.1.Through the background homozygous residual heterozygous line RHL32 derived from the hybrid combination of Pindong34(72-75g/TKW)and MY11847(20-25g/TKW),the correlation analysis of grain related characters showed that there was correlation among grain length,thousand grain weight and plant height.Using SSR markers and SNP markers to co-locate grains and other related traits on the 3D chromosome(95Mb-103Mb),the genetic map was constructed,the interval genes of grain weight and differentially expressed genes were functionally annotated,and the candidate genes were predicted.The reported Tasg-D1 gene located in 3D chromosome 95 Mb was cloned from Pindong34、 MY11847、RHL32B and RHL32 S,and the KASP molecular marker was developed,which is helpful to optimize grain shape by molecular marker-assisted selection.Increase the grain weight so as to increase the wheat yield and achieve the breeding goal of high yield.2.In this study,transcriptome analysis was performed on the grains of homozygous large grains and homozygous small grains in the remaining heterozygous line RHL32 derived from Pindong34 and MY11847 at 4 days,7 days and 10 days after anthesis,and differential genes were screened.The enrichment of differential genes GO and KEGG showed that the differential regulatory pathways between RHL32B(large grain)and RHL32S(small grain)were mainly oxidative phosphorylation,energy metabolism,photosynthesis,starch and sucrose metabolism pathway,GTP binding protein,material metabolism and transport.In addition,the differential genes were also enriched in the defense response pathway,and some genes related to grain weight and defense were mined,which laid the foundation for the study of the balance of wheat yield and disease resistance.3.In addition,Ta RPP13L1-3D,which carries out nuclear and cytoplasmic transport through interaction with Ran GAP-WPP domain,was taken as the research object to explore its functional intersection in yield and disease resistance.The detection of its expression pattern under the stress of wheat powdery mildew and stripe rust showed that it responded to the stress of powdery mildew and stripe rust.The subcellular localization of tobacco and onion epidermis cells showed that Ta RPP13L1 played a role in the nucleus and plasma membrane.Yeast two-hybrid experiment confirmed the direct interaction between Ta RPP13L1 and WPP1,and clarified the mechanism of RPP13L1 from cytoplasm to nucleus.Transient overexpression in wheat seedling stage and BSMV-RNA-induced gene silencing induced by tobacco transcription showed that Ta RPP13L1 gene was actively involved in wheat defense response to powdery mildew.Spike silencing of wheat materials using RHL32 B increased grain weight.In order to obtain stable genetic materials and explore the effects of Ta RPP13L1 on fungal stress response and grain development of wheat,we used CRISPR-Cas9 gene editing technique to edit Ta RPP13L1 gene in Bobwhite materials,and 5 mutants were screened in T0 generation. |
PDF Full Text Request