Research On Genes Regulated By Crop Salt Stress Based On Multi-species Transcriptome Analysis

Selection and breeding of salt-tolerant crops can effectively utilize saline soils and relieve pressure on arable land,as well as improve land salinization and reduce the negative effects of salt stress on crops.In order to better elucidate the salt tolerance mechanism of plants,this paper analyzes and compares different salt tolerance genotypes of closely related species and uses multi-species transcriptome analysis to reveal key genes for salt tolerance in crops.The paper addresses the problems of difficult and slow excavation of salt stress regulatory genes in rice and maize in traditional breeding,and conducts a study on the mining of key genes for crop salt stress based on multi-species transcriptome analysis.The specific research contents and results are shown as follows.1.The mining of differentially expressed genes under salt stress based on RNA-seq transcriptome analysis was carried out.After quality control,comparison and quantification,the number of differentially expressed genes under salt stress was found to be 7044(Arabidopsis thaliana),5218(rice)and 5349(maize)in three crops,respectively.GO and KEGG enrichment analyses of these genes were performed,and the results of these gene pathway annotations were found to be mainly focused on pyruvate metabolism,galactose metabolism,lysine degradation,and photosynthesis.These genes are closely related to the formation of cellular structures such as cell membrane and plasma membrane,and protein synthesis such as peroxisome in crops.2.Using Ortho Finder,Orthovenn2,953 direct homologous protein clusters of Arabidopsis,rice and maize were screened,133 pairs of single-copy homologous genes were screened,and protein interaction networks were constructed.Functional analysis of homologous protein clusters and single-copy homologous proteins was performed,and the annotation information indicated that most of the proteins were involved in transmembrane transport,secondary metabolic processes,response to reactive oxygen species The annotated information indicated that most of the proteins were involved in transmembrane transport,secondary metabolic processes,response to reactive oxygen species and response to salt stress,which supported the study of salt stress-related regulatory proteins.3.The WGCNA weighted gene co-expression network was used to analyze the gene expression patterns of two crops,rice and maize,respectively,and the genes with similar expression patterns were clustered.Finally,a total of 24 co-expression modules were obtained for rice and 17 co-expression modules were obtained for maize.Functional enrichment analysis of the modules was performed to obtain the core genes in the module networks,which can provide technical support for crop salt tolerance breeding.