Effects Of Acidifies Complex On Growth Performance,Serum Biochemistry Indicators And Fecal Microfloppies Of Weaned Piglets

To investigate the effects of dietary acidulant complex on the growth performance,serum biochemical indicators and intestinal microflora of weaned piglets.Ninety 28 days old healthy Duroc×Landrace×Yorkshire crossbred weaned piglets with a body weight of(8.0±1.0)kg were selected and randomly divided into three treatment groups:control group(CON),antibiotic group(AGP),and acidifier complex addition group(AC),Each group had 5 replicates and with 6 pigs per replicate.CON group was fed basal diet,AGP group was fed basal diet + AGP(bacitracin zinc 40 mg/kg,chlortetracycline 75 mg/kg,zinc oxide 2 kg/tonne),AC group was fed basal diet + 0.2 % Acidulant complex.The study lasted for 42 days.The study was divided into two phases(1-14 d and 15-42d)according to different diets.The results showed as below:1)Growth performanceIn 1-14 days,there was no significant difference in ADFI,ADG,and F/G among the groups.Compared with CON and AGP groups,AC group reduced the diarrhea rate(DR)by36.7%(P<0.01)and 48.2%(P<0.01).In 15-42 days,There was no significant difference in ADG among all groups(P<0.05),compared with CON group,ADFI and DR was reduced by 12.4%(P<0.05)and 61.4%(P<0.05)in AC group,respectively.In 1-42 days,ADFI of the CON group was significantly higher than that of the AGP group and AC group(P<0.05),and F/G of the AGP group was significantly lower than the CON and AC group(P<0.05).2)Serum biochemical indexesAt 14 and 42 days,there were no significant differences in T-AOC and MDA among all groups(P <0.05).At 21 d,compared with CON and AGP groups,T-AOC in AC group increased by 77.42% and 57.14(P <0.01),MDA decreased by 27.40% and 15.20%,respectively(P <0.05);During the whole trial period,there were no significant differences in serum related immune indexes among all groups(P <0.05).3)Fecal microbesThe addition of acidulant complex in the diet had no significant effect on chao1,observed＿species,PD＿whole＿tree,and shannon diversity index(P>0.05),but the shannon index of the AGP group was significantly increased by 13.3%(P<0.05)compared with the CON group.At the phylum level,Firmicutes,Bacteroidetes,Tenericutes,and Actinobacteria were the main dominant phyla,Adding acidifier compound had no significant effect compared with CON and AGP groups(P<0.05);At the genus level,the addition of acidulant complex significantly increased the relative abundance of Prevotella＿9(P<0.05)compared with the CON group;Compared with the AGP group,the addition of the acidulant complex could significantly increased relative abundance of Clostridium＿sensu＿stricto＿1(P<0.01)and Terrisporobacter,Intestinibacter,and Lachnospiraceae＿AC2044＿group(P<0.05).In summary,adding 0.2% the acidifier complex in the diet could significantly reduce the diarrhea rate of weaned piglets,improve the antioxidant capacity of weaned piglets in the second stage;and promot the growth and reproduction of intestinal beneficial bacteria,thus improve balanced intestinal of microflora.