Study On Harm Lessness Of Lipstatin Fermentation Residue And Effect Of Residue Organic Fertilizer On Soil Characteristics

Lipstatin fermentation residue is a solid waste produced during the production of orlistat by fermentation,which is rich in organic matter,nitrogen,phosphorus and potassium and other nutrients.In the process of resource utilization,a certain amount of lipstatin was left in lipstatin fermentation residue during the degradation process,which has potential risks to the environment and has been included in the hazardous waste management list of China in 2008.Lipstatin fermentation residue is rich in nutrients,but high water content and drug residues are the difficulties and key points of resource recovery of lipstatin fermentation residue,and it is urgent to establish an effective harmless and resource recovery way.To solve this scientific problem,this paper established a method for the detection of lipstatin in the residue and soil substrate,and compared three harmless treatment and disposal methods of lipstatin fermentation residue,including heat treatment,thermal hydrolysis treatment,aerobic composting method.After comprehensive analysis of the economic cost and development trend of the three treatment methods,aerobic composting method was selected as the optimal scheme for the harmless treatment of lipstatin residues.In addition,this study deeply analyzed the effects of lipstatin fermentation residue fertilizer on soil from the perspectives of soil physical and chemical properties,soil microbial activity,degradation of lipstatin in soil and bacterial abundance in soil,and analyzed the safety of environmental behavior of lipstatin fermentation residue(organic fertilizer)applied to soil after treatment.In order to lay a theoretical foundation for the practical application of lipstatin fermentation residue fertilizer.An ultra-high performance liquid chromatography-tandem mass spectrometry approach suited for the detection of the residual content of lipstatin in the fermentation residue was first devised in order to reliably assess the residual content of lipstatin in the fermentation residue and soil.Acetonitrile was employed as the extractant,5 m L of extractant was extracted twice,and the two supernatants were mixed as a technique of extracting lipstatin from fermentation residue.The recovery rate was satisfactory,the method’s limit of quantification was 10 μg/kg,and the linear relationship was good,indicating that the approach could be utilized to detect residual lipstatin in fermentation residues.The ultrasonic-assisted extraction approach was also used to identify lipstatin in soil.1 m L sodium chloride*calcium chloride solution and 5 m L acetonitrile were used as the extraction agent,and ultrasonic-assisted extraction was conducted once.The recovery rate was satisfactory,the method’s limit of quantification was 10 μg/kg,the linear relationship was satisfactory,and it could be utilized as an extraction method for detecting lipstatin fermentation residue in soil.The above-mentioned approach was employed to extract the leftover lipstastin in the fermentation residue,and three benign processes were compared.The thermal treatment method at 250 °C for 30 minutes degraded the leftover lipstatin in the fermentation residue;the thermal hydrolysis method at 160 °C and after 180 minutes,the lipstatin degradation rate reached more than 90%,according to the findings.The initial C/N of aerobic composting was 20 in the experiment,and the moisture content was 60%.The residual lipstatin in the compost was close to 100% after 30 days of aerobic composting.The aerobic composting method was chosen as the harmless method of lipstatin fermentation byproduct disposal due to its costeffectiveness and safety.The environmental behavior of composted fungal wastes applied to the soil,as well as its effect on soil parameters,were studied in a laboratory soil incubation experiment.The physicochemical features of the soil,as well as its microbial activity and bacterial abundance,were studied in detail.The findings revealed that when organic fertilizer was added to the soil,it significantly enhanced the amount of accessible phosphorus and potassium in the soil while having no effect on the enzymes in the soil.It had a good effect on soil microorganisms in the usual range,and increasing soil salinity could boost soil system stability.After 30 days of testing,no residual lipstatin was found in any of the experimental group’s samples,and the addition of fermentation byproduct organic fertilizer boosted the number of soil actinomycetes and bacteroidetes,as well as the diversity index.The results revealed that applying lipstatin fermentation leftover organic fertilizer did not have a detrimental impact on soil qualities,in fact,they tended to improve them.The preceding tests shown that the aerobic composting approach may be used to safely dispose of lipstatin fermentation waste while also maximizing resource use.