Regulation Of Growth And Cold Tolerance Of Maize Seedlings Under Low Temperature Stress Induced By Gongzhulingmycin

Maize plays an important role in China’s agricultural production.Low temperature a nd cold damage during maize germination and seedling growth period will affect maize yield and lead to yield loss.Gongzhulingmycin has certain regulation effect on crop gro wth and stress resistance.In this study,maize was used as the target crop to determine the substrate of maize,the optimal concentration of Gongzhulingmycin,and the changes of growth phenotype,gene expression and enzyme activity of maize in germination and seedling stages under low temperature stress induced by Gongzhulingmycin.The experimental results show that:(1)In laboratory simulated environment,hydroponic cultivation was more suitable to investigate the changes of maize growth traits induced by Gongzhulingmycin in labor atory simulated environment.The comprehensive growth traits of maize seedlings were b etter when they were planted with 500X～1000X water extract of Gongzhulingmycin.Wi th 1000 X diluted Gongzhulingmycin water extract hydroponics,maize germinated and gre w at constant low temperature of 6℃,10℃ and 14℃,respectively.Comprehensive gro wth traits are best performed at 14°C.(2)Under low temperature growing environment,Gongzhulingmycin can promote the comprehensive growth phenotype of maize at germination and seedling stage to enha nce cold resistance.(3)Gongzhulingmycin induced increased the response of cold responsive gene Zm NAP1-q and antioxidant gene CAT3 to low temperature stress.(4)The initiation level of antioxidant enzymes in maize embryos was changed byGongzhulingmycin induction.Under the continuous low temperature environment,the ac tivity of antioxidant enzymes in the seed embryo during germination was induced by Go ngzhulingmycin,which made the seed more conducive to low temperature stress.