| Lycium barbarum L.is the source plant of traditional Chinese medicine wolfberry,which is mainly distributed in Ningxia,Gansu,Qinghai,Xinjiang and other northwest provinces.Environmental difference is an important factor for organisms to differentiate into new species,and selective elimination can effectively identify the selected significant regions.In this paper,based on the whole genome resequencing data of Lycium barbarum L distributed in Qinghai,Xinjiang and Ningxia,three selective elimination analysis methods were used to identify the candidate genes of genome differential differentiation and response to the accumulation of effective components among Lycium barbarum L populations in Ningxia,in order to provide theoretical support for revealing the genuine causes of Lycium barbarum L.The main conclusions of this paper are as follows:1、A total of 33.52 million SNP loci were detected by weight sequencing of Lycium barbarum L,which greatly enriched the SNP information of Lycium barbarum L population in Ningxia.The SNP variation sites were unevenly distributed in the genome,of which 25.51%were located in introns and 3.51%in exons.The analysis of population structure showed that the population in Qinghai was relatively independent,and it was difficult to separate the individuals in Ningxia and Xinjiang.Among the three selective removal methods(Fst,Pi,XP-CLR),the number of genes screened by XP-CLR method was significantly larger than that of Fst and Pi.In the overlapping region,810 genes were screened in Ningxia and Qinghai,363 genes were screened in Ningxia and Xinjiang,and 202 genes were screened in Qinghai and Xinjiang.Among them,there are more selected genes in chr06,and the selected genes on chr10 are more significant.In the three regions,the degree of genetic differentiation within populations was low,and the genetic differentiation among populations was obvious.Lycium barbarum L in Qinghai area has obvious differentiation and low genetic diversity,and Lycium barbarum L gene exchange between Ningxia and Xinjiang is frequent.2、A total of 111 metabolic pathways were located in the overlapping region.The significantly enriched regions mainly included enzyme activity expression pathways,DNA synthesis pathways,lipid synthesis and metabolic pathways related to physiological processes.These pathways were closely related to cell cycle,photosynthesis,DNA processing and modification,energy production and transformation,amino acid transport and metabolism,ribonucleotide synthesis and metabolism,carbohydrate transport and metabolism,lipid transport and metabolism,signal transduction mechanism,amino acid synthesis and metabolism,secondary metabolite biosynthesis.It shows that Lycium barbarum L will change its own oxidoreductase activity,change the expression of functional genes involved in its metabolism,energy synthesis,protein post-translational modification,cell signal transduction and other functional genes in response to environmental stress.3、The results of gene expression were confirmed by transcriptome data.Finally,10 related pathways and 128 related genes were identified.Two related pathways and eight related genes were identified.Three related pathways and 32 related genes related to betaine synthesis were mapped.A total of 6 genes related to the accumulation of active ingredients were obtained,including ALDH,G6DP,SUS,E3.1.1.11,E3.2.1.14 related to polysaccharide synthesis and CHS related to flavonoids.4、There are differences in the content of active ingredients in Lycium barbarum L in different regions.The content of polysaccharides and flavonoids in Lycium barbarum L in Qinghai is the highest,and the content of betaine in Xinjiang is higher.In each region,the annual average temperature,annual active accumulated temperature and the highest temperature in July are the main climatic factors,and the content of elements that can be directly absorbed in the soil is the main soil factor.LBP was significantly negatively correlated with annual average temperature,the highest temperature in July,and annual active accumulated temperature,and significantly positively correlated with annual sunshine duration.The content of flavonoids was significantly negatively correlated with the annual average temperature,the highest temperature in July and the annual active accumulated temperature,significantly negatively correlated with the annual average humidity,and significantly positively correlated with the annual sunshine duration.The content of betaine was significantly positively correlated with the total phosphorus content of soil.In this study,the variation of Lycium barbarum L in Ningxia was explored from the whole genome level,and the candidate genes in response to environmental changes and the genes related to the accumulation of active ingredients were screened by combined analysis of three selective removal methods.It provides an important theoretical basis for excavating the unique genetic information resources of Lycium barbarum L in different regions and exploring the molecular mechanism of effective component accumulation in response to environmental changes. |
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