Regulation Of Light Quality On Physiological Metabolism Of Acanthopanax Senticosus(Rupr.&Maxim.) Harms Leaves

Acanthopanax senticosus(Rupr.& Maxim.)Harms is a true medicinal material in northeast China,and its roots,stems,and leaves can be used as drugs.Long-term use has the effect of lightening the body and prolonging life.In recent years,the A.senticosus artificial cultivation industry has reached a certain scale.Improving its quality and yield while ensuring the large-scale promotion of artificial cultivation has become a important topic in the field.In this study,annual A.senticosus seedlings were used as the material,and LED white light was used as the control group(CK).Four kinds of LED red and blue substances with different proportions were set as the treatment group: red/blue = 2/1(2R1B),red/blue = 3/1(3R1B),red/blue = 5/1(5R1B),and red/blue = 7/1(7R1B).By measuring and analyzing the growth indicators,physiological characteristics,and metabolites of A.senticosus treated with different proportions of red and blue composite light,we systematically explored the response mechanism of the physiological metabolism of A.senticosus to the regulation of different proportions of red and blue pigments.The following conclusions were reached:(1)5R1B was the most beneficial for the growth of A.senticosus,followed by 7R1 B.7R1B was most conducive to the accumulation of chlorophyll in A.senticosus.Both 3R1 B and5R1B significantly improved the photosynthetic capacity of A.senticosus leaves.2R1 B and3R1B significantly increased the activity of antioxidant enzymes,and 5R1 B significantly increased the contents of total phenols and total flavonoids in leaves of A.senticosus.5R1 B and 7R1 B treatments significantly increased soluble sugar content;3R1B and 5R1 B treatments significantly increased soluble protein content.(2)In this study,a total of 52 different compounds,including sugars,amino acids,organic acids,and alcohols,were screened using GC-MS non-targeted metabolomics technology.Through the enrichment analysis of metabolic pathways,eight different metabolic pathways were identified: pentose phosphate pathway,carbon fixation,alanine,aspartic acid and glutamate metabolism,glyoxylic acid and dicarboxylic acid metabolism,citrate cycle(TCA cycle),galactose metabolism,sulfur metabolism,and glycine,serine,and threonine metabolism in photosynthetic organisms.Red and blue composite light significantly promoted the primary metabolism of A.senticosus leaves,with 5R1 B treatment significantly promoting carbohydrate metabolism,amino acid metabolism,and energy metabolism of leaves.(3)In this study,UPLC-MS technology was used to determine the secondary metabolites in A.senticosus leaves treated with different light qualities,and a total of 19 phenolic compounds were identified.Cluster analysis showed that most of the 19 target phenolic compounds had high accumulation in the 2R1 B,3R1B,and 5R1 B treatment groups.The CK group promoted the accumulation of L-phenylalanine,while the 3R1 B treatment group promoted the accumulation of C6C1 phenolic compounds,and the 2R1 B treatment group promoted the accumulation of C6C3 phenolic compounds.The 5R1 B treatment promoted the accumulation of C6C3C6 phenolic compounds.The study determined a total of five active substances in the leaves of A.senticosus spp.treated with different light qualities:Eleutheroside B,Eleutheroside E,hyperoside,isoazinpyridine,and oleanolic acid.Among the treatments,2R1 B significantly promoted the accumulation of active components in A.senticosus leaves per unit mass.The yield of Eleutheroside B,Eleutheroside E,and isoazinpidine reached the highest under the 5R1 B treatment,while the yield of hypericin and oleanolic acid reached the highest under the 2R1 B treatment.