Protection Effect Of Hydrogen On Oxidative Skin Damage And Trauma In The Dairy Teat

The dairy industry is an important part of China’s livestock industry and plays an important role in the economic development of China.Teat injury has a large impact on milk production of cows and threatens the healthy development of the dairy industry.The harsh winter weather conditions and the frequent use of baths are the main factors causing teat damage in dairy cows.Therefore,the problem of cow teat damage in dairy farms needs to be taken seriously.In recent years,with the continuous research on hydrogen,it has been found that hydrogen has selective antioxidant effects by scavenging toxic free radicals,and also has significant therapeutic effects in promoting wound healing.Hydrogen has therefore become a therapeutic gas with great potential for development.Given the therapeutic properties of hydrogen,this study investigated the protective effects of hydrogen on oxidative damage and trauma in cow teat skin through MAC-T cells and KM mouse models and clinical trials in Holstein cows.To investigate the effect of hydrogen on the oxidative stress response of MAC-T cells induced by povidone-iodine(PVP-I),the cells were randomly divided into control group(Control),hydrogen group(H2),oxidative stress group(PVP-I),and oxidative stress +hydrogen group(PVP-I + H2)in this experiment.The first step was to clarify the effect of PVPI on cells,firstly,MAC-T cells were treated with different concentrations of PVP-I for 2h to establish an oxidative damage model,and the cell viability was measured at different concentrations using CCK8.According to the degree of cell viability change,the experimental conditions of PVP-I concentration of 12.8 mmo L and treatment time of 2 hours were selected for this experiment.Next,the cells were treated with hydrogen-enriched cell culture solution for 2h to detect the changes of relevant indexes.The expression of intracellular endogenous factor-related factor 2(Nrf2)and heme oxygenase 1(HO1)were detected by protein immunoblotting(Western Blot).Analysis of intracellular levels of reactive oxygen species ROS and cell death after stimulation by PVP-I by flow cytometry.Changes in malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione reductase(GSH)were also measured with the kit.Based on the experimental results,it was concluded that hydrogen alleviated the decreased activity of MAC-T cells caused by PVP-I,reduced the accumulation of intracellular ROS,and inhibited the oxidative stress caused by PVP-I.It indicates that hydrogen alleviates the oxidative damage of MAC-T cells induced by PVP-I by maintaining the balance of redox system.In order to evaluate the application of hydrogen,this study prepared hydrogen-containing glycerol as a hydrogen-rich carrier to increase the permeability to the skin,and further explored the protective effect of hydrogen on oxidative damage and trauma of cow teat skin through a mouse skin trauma model and a clinical trial in cows.First,30 mice were randomly divided into three groups: control group(Control),glycerol group(Gly),and hydrogen-rich glycerol group(HRG).The treatment was applied to the skin wounds,once in the morning,once in the afternoon and once in the evening,for one week,and the skin healing status was observed.Based on the results of skin healing rate comparison,it was concluded that hydrogen rich glycerin application accelerated the healing of skin wounds in mice.At the same time,30 Holstein cows were divided into three groups: the medicated bath solution group(PVP-I),the medicated bath solution + glycerol group(HRG + PVP-I),and the medicated bath solution +hydrogen-rich glycerol group(PVP-I + HRG).The treatment was applied to the cow’s teats,once in the morning,once in the afternoon and once in the evening,for two months,and then the condition of the cow’s teat skin was observed.Based on the comparison of skin condition between the groups,it was concluded that the teat skin folds were reduced and the surface was smoother in the hydrogen-rich glycerol group,indicating that hydrogen-rich glycerol could reduce the oxidative damage to the teat skin caused by the bathing solution.In conclusion,hydrogen could alleviate the oxidative damage of MAC-T cells induced by PVP-I by maintaining the balance of redox system,and hydrogen-rich glycerol could promote the healing of skin wounds in mice as well as improve the skin condition of teats of cows induced by medicinal bathing solution.This study demonstrated the value of hydrogen for clinical applications in animals with oxidative skin damage and trauma,and laid the foundation for the application of hydrogen in animal husbandry.