Effects Of Different Macroelements On Plant Phenotype,Flavonoid Metabolism And Transcriptome Analysis Of Erigeron Breviscapus

Erigeron breviscapus [Erigeron breviscapus(Vant.)Hand.-Mazz] is also known as Erigeron breviscapus,Erigeron breviscapus,Asarum breviscapus,Asarum breviscapus,Asarum breviscapus,Asarum breviscapus,Asarum breviscapus,Asarum breviscapus,Nelumbo nucifera,Nelumbo nucifera,Asteraceae.It has the effects of promoting blood circulation and removing blood stasis,dredging meridians and activating collaterals,dispelling cold and relieving exterior,dispelling wind and removing dampness,anti-inflammatory and analgesic.A large number of pharmacological studies have shown that the main active ingredient in Erigeron breviscapus is the total flavonoids of breviscapine,and its content is related to the large number of elements in the growth environment,but its mechanism is not clear.In this experiment,the new seedlings of Erigeron breviscapus were used as experimental materials under greenhouse soil cultivation conditions.Based on the MS Base Salts(-N-P-K,with vitamins)medium produced by Coolaber,a three-factor and three-level orthogonal design was carried out,a total of 9 treatment groups.The plant phenotype under different treatments,the changes of plant elements in cultivated soil,the chlorophyll fluorescence parameters of leaves were measured,and the transcriptome of the above-ground parts of 9 treatments at seedling stage and flowering stage was measured and analyzed.The results show that :1.The combination of different elements had a significant effect on the stomata of Erigeron breviscapus leaves.The stomatal opening and closing degree of YE11 was lower,and the stomatal opening and closing degree of YE15 and YE19 was higher.It shows that the stomatal opening degree of Erigeron breviscapus has a certain relationship with nitrogen,phosphorus and potassium.Excessive nitrogen will reduce the stomatal opening degree,high potassium will increase the stomatal opening degree,and high phosphorus can alleviate the low stomatal opening degree caused by high nitrogen.2.The combination of different elements had a significant effect on the phenotype of Erigeron breviscapus plants.Among them,YE12,YE16 and YE19 had better growth,large plant morphology and strong root development.It shows that high phosphorus is beneficial to the growth and root development of Erigeron breviscapus,while nitrogen should not be too high.Appropriate reduction of potassium can alleviate the adverse effects of high nitrogen on plants.3.The effects of different element combinations on the element content of cultivation matrix and leaves were significantly different,and changed with time.For example,on the 10 th day,the soil ammonium nitrogen content of YE11 and YE19,the soil available phosphorus of YE11 and YE13,and the soil available potassium of YE11 and YE13 were significantly different.There were significant differences in leaf nitrogen content between YE13 and YE17,phosphorus content between YE11 and YE16,and potassium content between YE15 and YE18.It shows that a large number of elements in the cultivation matrix will affect the element content in plant leaves.4.The effects of different element ratios on the content of flavonoids in the leaves of Erigeron breviscapus were different.At 2/3N and 1/1N,the content of flavonoids increased significantly,indicating that high nitrogen will reduce the total flavonoids of Erigeron breviscapus.5.The effects of different element treatments on chlorophyll fluorescence parameters of Erigeron breviscapus were very significant,among which F0 of YE11-YE14,YE11-YE15 and YE11-YE16,Fm of YE11-YE16 and YE11-YE17,Fv /Fm of YE11-YE17,YE13-YE14 and YE13-YE17,NPQ of YE11-YE16,YE11-YE17 and YE11-YE19,q P of YE11-YE15,YE11-YE16 and YE12-YE15 were significantly different.The results showed that the minimum initial fluorescence(F0)of Erigeron breviscapus leaves under total N showed an increasing trend,while the maximum fluorescence(Fm)and maximum photochemical quantum efficiency(Fv / Fm)showed a decreasing trend.6.Based on transcriptome data,through GO enrichment analysis,it was found that there were great differences in annotated genes with different functions,among which oxidoreductase activity,response to water deprivation,photosynthetic membrane,chloroplast thylakoid,photosynthesis and so on.The co-enrichment of 20 signal channels by KEGG found that different element combinations had effects on different channels.Among them,COMT,peroxidase,CYP73 A and 4CL in the phenylpropanoid biosynthesis pathway were the most influential;c HS,CHR,F3 H in flavonoid biosynthesis pathway;n RT,NR,nirA,CA,GLU in nitrogen metabolism regulation pathway.In summary,different element combinations had different effects on stomatal,plant phenotype,flavonoid content and chlorophyll fluorescence parameters of Erigeron breviscapus leaves.Among them,nitrogen had the most obvious effect on flavonoid content,and there was interaction between elements.Transcriptome analysis also showed that COMT,peroxidase,CYP73 A and 4CL in phenylpropanoid biosynthesis pathway,CHS,CHR and F3 H in flavonoid biosynthesis pathway were treated with different elements.NRT,NR,nirA,CA and GLU were all affected in the regulation pathway of nitrogen metabolism.The results showed that the combination of different elements may be mediated by nitrogen utilization,thus affecting flavonoid metabolism.The experimental conclusions have a good role in further elucidating the mechanism of the influence of nutritional environment factors on the synthesis of flavonoids.