Development And Effect Evaluation Of Active Bio-organic Fertilizer In Tobacco-growing Soil

Declining soil activity for tobacco planting is one of the main factors limiting the healthy development of my country’s tobacco industry.In this study,25 strains of growth-promoting bacteria preserved in the laboratory were selected,and the growth-promoting ability of the strains was evaluated by the entropy weight method,and 4 functional strains with significant growth-promoting effects were selected and prepared into different biological organic fertilizers;the growth-promoting effects were screened out through pot experiments Two kinds of remarkable bio-organic fertilizers;through field experiments,the two kinds of bio-organic fertilizers have been analyzed to promote the growth of tobacco and improve the soil activity of tobacco planting,and obtain one kind of bio-organic fertilizer that can significantly improve the soil activity of tobacco planting.The results of the study are as follows:1.Determination of growth-promoting indexes and screening of growth-promoting strains.The main growth-promoting indexes(nitrogen fixation,HCN production,phosphorus dissolution,potassium dissolution,and IAA secretion ability)of 25 strains stored in the laboratory were determined.The results showed that all 25 strains had growth-promoting effects,among which strain W1 had significant nitrogen fixation,Potassium-dissolving ability.Strain F2 has remarkable ability to produce HCN,potassium-dissolving and long hormone.Strains VC48 and LX5 have remarkable ability to produce HCN and auxin.At the same time,25 strains were found to promote tobacco seed germination and seedling growth.,Can be used as the starting strain for the next step in the development of bio-organic fertilizer to improve the activity of tobacco-growing soil.2.The entropy method comprehensively evaluates the growth-promoting strains.Through the root irrigation test,the entropy weight method was used to comprehensively evaluate the effect of 25 strains on the promotion of tobacco growth.The results showed that the strains W1,F2,VC48 and LX5 had significant rhizosphere growth promotion effects;through the antagonism test,it was shown that the strains W1 and F2 There is an obvious antagonistic effect between LX5 and VC48,but there is no antagonistic effect between F2and VC48.Therefore,four kinds of bio-organic fertilizers were prepared by fermentation of strains W1,F2,VC48 single bacteria and F2 and VC48 mixed bacteria respectively.3.Potted plant experiment to screen the growth-promoting bio-organic fertilizer.The results showed that the four bio-organic fertilizers all have the effects of improving tobacco agronomic properties,soil physical and chemical indicators,soil enzyme activities,and soil microbial diversity.Among them,the application of inorganic fertilizer+soil-improving organic fertilizer+bio-organic fertilizer prepared by F2 and VC48 mixed bacteria fermentation(PT4)tobacco’s root fresh weight,aboveground fresh weight,maximum leaf width,maximum leaf length,plant height,stem diameter and The number of effective leaves was significantly higher than that of other treatments,which were 58.55%,25.92%,22.26%,21.18%,33.12%,44.67%and 11.38%higher than the application of inorganic fertilizer+soil-improving organic fertilizer treatment(PCK3);Fertilizer+soil-improving organic fertilizer+bio-organic fertilizer treatment(PT1)prepared by fermentation of strain W1increased the activities of invertase,urease and catalase in tobacco-growing soil compared with the treatment of inorganic fertilizer+soil-improving organic fertilizer(PCK3).71.22%,8.53%and 49.90%,indicating that the application of inorganic fertilizer+soil-improving organic fertilizer+bio-organic fertilizer treatment(PT4)prepared by mixed fermentation of F2 and VC48 and application of inorganic fertilizer+soil-improving organic fertilizer+W1strain The bio-organic fertilizer treatment(PT1)prepared by fermentation can significantly promote the growth of tobacco and improve the activity of the tobacco-growing soil.4.Field experiments verify the effect of bio-organic fertilizer on improving soil activity.The results showed that the bio-organic fertilizer treatment(T2)prepared by the application of inorganic fertilizer+soil-improving organic fertilizer+F2 and VC48 mixed bacteria fermentation had a significant effect on the improvement of tobacco agronomic characteristics.After 75 days of transplanting,the total root length of tobacco plants treated with T2 was The total root surface area,average root diameter,and total root volume were significantly higher than other treatments,which were 88.35 m,1996.09 cm~2,2.16 mm,and36.96 cm~3,respectively;through the analysis of soil microbial community diversity,the soil bacterial biodiversity of T2 treatment was significant The abundance of Actinomycota is higher than other treatments and lower than other treatments.It shows that the application of inorganic fertilizer+soil-improving organic fertilizer+bio-organic fertilizer prepared by F2and VC48 mixed bacteria fermentation in the field experiment can significantly improve the growth characteristics of tobacco and increase the soil activity of tobacco planting.In summary,this study started from 25 preserved strains,through the evaluation of growth promotion ability,pot experiment screening,and field effect verification.The results show that under the premise of the same level of nitrogen application,Guizhou tobacco area can use inorganic fertilizer+organic fertilizer.+The comprehensive fertilization program of bio-organic fertilizer improves the activity of tobacco-growing soil,namely:inorganic fertilizer(special compound fertilizer for tobacco)+soil-modified organic fertilizer(organic fertilizer for mushroom residue)+bio-organic fertilizer fermented by mixed bacteria of F2and VC48.