Study On The Regulation Method Of Embryonic Hatching Cycle Of Takifugu Obscurus Based On Effective Accumulation Temperature Model

As a freshwater aquaculture species with fresh meat and high medical value of its internal secretion,dark oriental triggerfish occupies an important role in the aquaculture industry with a promising comprehensive utilization.In the fish farming industry,suitable incubation temperature is a crucial factor,which directly affects the development speed and hatching quality of fish embryos.By maintaining sufficient and high quality embryos during the hatching process,the productivity and quality of fish farming can be effectively improved.There is no doubt that embryo hatching is the beginning of the whole chain and also guarantees the smooth running of the subsequent chain.If the hatching environment is unsuitable,it will lead to abnormal embryo development,prolonged hatching cycle and reduced hatching rate,resulting in insufficient litters for subsequent breeding work,which will greatly affect the fish culture industry.Therefore,in order to ensure the sustainable development of fish farming industry,it is necessary to pay attention to the regulation and management of hatching temperature to ensure the adequate hatching of fish embryos and thus improve the output and quality of fish farming.In order to meet the on-time,quality and quantity production of dark oriental fish embryo hatching,a technique of dark oriental fish embryo hatching cycle regulation based on the effective cumulative temperature model H=78.905/(T-7.6033)is proposed.The thesis focuses on the following aspects.(1)Based on the characteristics of intensive artificial breeding of dark oriental triggerfish,the breeding area is divided into temperature regulation and embryo hatching area.This can isolate the process of water quality regulation from embryos and avoid the negative impact of large changes in water quality on the hatching process of embryos.An image recognition system for embryo hatching rate and embryo growth stages was also designed using YOLO v5 s.By examining the sampled embryos with electron microscopy,together with the image processing technology of artificial intelligence,the embryo development stages are classified and the hatching rate and mortality rate of embryos are counted to guide the production process.(2)This thesis summarized the effect of incubation environment temperature on the hatching cycle of dark oriental triggerfish embryos,and proposed a scheme to derive the target temperature at incubation using the effective cumulative temperature model and the target production cycle.Meanwhile,how to combine the target hatching cycle to derive the target hatching temperature was discussed.(3)After getting the target hatching environment temperature,the fuzzy PID algorithm is used to control the flow of groundwater of both hot and cold temperatures into the buffer pool in the water quality regulation area.Through the heat exchange of water at different temperatures,the hatching water is introduced into the embryo incubation area by the constant pressure water tower after reaching the set target temperature.By conducting simulation experiments,the performance differences between traditional PID control and fuzzy PID control algorithms were compared,and the appropriate regulation method was selected.The results show that the fuzzy PID algorithm has obvious advantages in the water temperature regulation system of dark oriental trigger embryos,which can converge to stability faster and basically without overshoot,meeting the system requirements.According to the actual production situation,the target incubation environment temperature of 20.75℃ that can complete the incubation of dark oriental triggerfish embryos within 6 days was determined under the theoretical guidance of the system.In order to ensure the health and growth and development of embryos,the system adopted a buffer pool for water quality control,which effectively avoided embryo deformation caused by abnormal changes in water temperature and improved embryo hatching rate and embryo survival rate.In addition,combined with artificial intelligence image classification technology,the system can classify the embryo development stages during the hatching process and count key indicators such as hatching rate and mortality rate,thus ensuring the production target of hatching rate above 92% with high activity and low deformation rate.In conclusion,this dark oriental triggerfish embryo hatching system has a good ability to control the hatching cycle,which is conducive to the normal development of subsequent breeding work and provides a reference for the construction and promotion of efficient hatching models for other aquaculture species.