| Rhizosphere microorganisms have a significant impact on plant growth,as they can promote plant growth;It can decompose organic matter and minerals,release nutrients,and improve the soil environment.Rotation can have various impacts on rhizosphere microorganisms.The planting of different crops can lead to changes in environmental factors such as nutrients,p H,and water content in the soil.Rotation can make soil ecosystems more complex and diverse,increasing the presence and interaction of different types of rhizosphere microorganisms in the soil,thereby improving the stability and sustainability of soil ecosystems.These changes may directly or indirectly affect the quantity and composition of rhizosphere microorganisms.In recent years,the development of metagenomic technology has enabled us to directly extract all microorganisms in soil for sequencing analysis.We can obtain the specific species composition differences and functional characteristics of microbial communities among sample groups.This article mainly uses metagenomic second-generation sequencing technology to analyze and study the rhizosphere microorganisms of spring crops(faba beans,quinoa,barley)under water drought rotation(rice small spring crop).Using spring crops(faba beans,quinoa,barley)under drought drought rotation(tobacco small spring crop)as a control,we study the differences in the composition and abundance of rhizosphere microorganisms between them,thereby affecting the differences in agronomic traits between small spring crops,Select the types of dominant crops under water drought rotation for spring and spring crops.The following results were obtained from the work of this article:1.In terms of agronomic traits,there is no significant difference in various agronomic traits between the two different rotations of barley.However,in terms of yield,the barley under water drought rotation is 15% higher than that under drought drought rotation;Among quinoa,the yield of quinoa under drought drought rotation is significantly higher than that under drought rotation,with a yield increase of 19%;In broad beans,there is no significant difference in various agronomic traits and yield between two different rotations of broad beans.2.In soil physico-chemical properties,the content of hydrolyzed nitrogen and available potassium in rice crop rotation was significantly higher in quinoa samples than in barley crop in drought crop rotation.In barley samples,the content of hydrolytic nitrogen and available potassium in rice crop rotation was relatively high,and the content of chlorine ion in barley spike was significantly lower than that in dry crop rotation.In broad bean samples,the content of hydrolytic nitrogen in the soil of broad bean treated with rice rotation was significantly higher,but the effective phosphorus content in the pod-bearing soil of broad bean was lower.3.By means of metagenomic analysis,a comparison of species diversity analysis revealed that the most significant difference between broad bean sample groups was planctomyces at the genus level compared to dry and dry rotations.Planctomyces and Roseimicrobium were the most significant differences in barley samples.The most significant difference in the quinoa sample group was in the genus Roseimicrobium.At the seed level,the most significant differences in the quinoa sample group were in the polar worm monomonas E3S(Polaromonas＿sp.＿E3S),in the broad bean sample group Dyadobacter＿fermentans,and in the barley sample,the lake Nitrospira＿lacus.4.Gene annotation and differential gene analysis of rhizosphere microorganism showed that most of the differential genes were related to mitosis in barley samples.Among broad bean samples,some of the genes were related to fatty acid and lipid oxidation,and some genes were related to organic synthesis.Among quinoa sample groups,most of the differentially expressed genes were associated with nucleotide metabolism and damage and repair of nucleotide.5.By comparing agronomic characters,soil physico-chemical indexes and metagenomic analysis of three small spring crops,the best planting patterns of the small spring crops were: tobacco-quinoa,rice-broad bean and rice-barley. |
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