Analysis Of Important Functional Genes Of Huamoxiang 5 And Breeding Improvement Design

Rice(Oryza sativa L.)is one of the model plants for functional genomics research.Currently,researchers have discovered and cloned over 4,400 functional genes in rice,and they have found genetic and functional differences in several important functional genes among different germplasm populations.In response to the increasing demand for rice flavor and food safety,researchers have developed a rice variety called Huamoxiang 5,which is a whole-grain aromatic rice.Based on the whole-genome sequencing of Huangmoxiang 5,this study aims to analyze Huangmoxiang 5 at the functional genomic level and develop an improvement plan for Huangmoxiang 5.Taking Huangmoxiang 5 as an example,the study compiles a rapid alignment analysis method for functional genes,summarizes the information of important functional genes in rice,and constructs a database of important functional genes in rice.In this study,a total of 245 important functional genes related to disease resistance,insect resistance,nitrogen and phosphorus efficiency,stress tolerance,heavy metal resistance,grain quality,heading date,and yield were identified and analyzed.Through literature review,it was found that 20 genes did not have specific functional variation sites,so a total of 225 important functional genes in rice with 342 gene variation sites were compiled to construct the database of important functional genes in rice.Taking Huangmoxiang 5 as an example,this study identified and analyzed 245 important functional genes.The location information of each gene in Huangmoxiang 5 was obtained through the rapid alignment analysis method for functional genes,and the presence or absence of each gene in the genome was determined.Furthermore,based on the gene variation sites or alignment consistency information,the gene functions were quickly identified.Through analysis,86 advantageous allele genes related to biotic and abiotic resistance,nitrogen and phosphorus efficiency,yield,and grain quality were identified in Huangmoxiang 5,along with 134 non-advantageous genes,19 growth heading date genes,and 6 male sterility genes in indica-japonica hybrid.This study analyzed the target traits of green super rice production,analyzed the relevant functional genes in Huangmoxiang 5,and developed an improvement plan for functional genes in Huangmoxiang 5,combining with the germplasm resources available.Additionally,a rapid alignment analysis method for functional genes was compiled,225functional gene variation sites were summarized,and a database for breeding important functional genes in rice was constructed,providing a theoretical basis for functional genomics-based breeding.It also serves as a paradigm for subsequent improvement of backbone parental lines in functional genomics.