| Objective:1Formulate medium-and long-term aerobic exercise program for rats and construct medium-and long-term aerobic exercise rat model.kTo elucidate the change rule of aerobic exercise on serum metabolism of model rats,and to enrich and determine the influence of metabolic pathways.lTo determine metabolic markers and explore the predictive value of metabolic markers.Methods:In this study,a randomized controlled trial was conducted.According to the random number table method,6-week-old SD rats were randomly divided into aerobic exercise group(experimental group)and atherosclerosis rat model group(control group).SD rats were specially fed with high nutrition diet with high sugar,high fat and high cholesterol.At the 24 th week,samples were collected and analyzed from the oxygen exercise group(experimental group)and the atherosclerosis rat model group(control group),and the experimental data were recorded: body weight,blood glucose and lipid indexes of the rats,and the results of oil red O staining and HE staining sections of the arterial tissues of the rats were observed.Statistical analysis was performed using SPSS 25.0.Measurement data were expressed by means of mean and standard deviation,and inter-group and intra-group comparisons were made for the experimental data of the two groups.The independent sample t-test was used for the comparison at the same time between groups,and the paired t-test was used for the comparison before and after the comparison within groups.The test level was set at 0.05,and P<0.05 was considered statistically significant.Sera of the two groups were analyzed by metabonomics to determine their influence pathways and metabolic markers.Results:1.At the 24 th week,the survival rate of medium-and long-term aerobic exercise rat model was 100%.2.Changes in body weight: there was no significant difference in body weight between the two groups before the experiment(P=0.503);After 24 weeks of aerobic exercise intervention,the weight of rats in the aerobic exercise group was significantly lower than that in the model control group,and the difference was statistically significant(P=0.000).3.Changes in blood glucose: there was no significant difference in blood glucose between the two groups before the experiment(P=0.701).After 24 weeks of aerobic exercise intervention,the blood glucose of rats in the aerobic group was significantly lower than that in the model control group,and the difference was statistically significant(P=0.000).4.Changes in triglycerides: there was no significant difference in triglycerides between the two groups before the experiment(P=0.805);After24 weeks of aerobic exercise intervention,the triglyceride of rats in the aerobic exercise group was significantly lower than that in the model control group,and the difference was statistically significant(P=0.000).5.KEGG enrichment pathways: Anion enrichment pathways are mainly arachidonic acid metabolism(P=0.008)and unsaturated fatty acid biosynthesis pathway(P=0.022).The cation enrichment pathways were mainly pyrimidine metabolism(P=0.013)and sodium reabsorption regulated by aldosterone(P=0.033).The differences were statistically significant(P <0.01).6.According to the enrichment pathway,the marker metabolites in aerobic exercise were screened out:VIP >1.419,FC > 6.700 and P value=0.000 for arachidonic acid,VIP for cytoline =1.553,FC =16.963 and P value=0.000 and cortisone VIP > 1.562 for arachidonic acid,FC >53.169 and P value=0.000.Conclusion:1.The exercise program in this study can build a rat model of medium-and long-term aerobic exercise.2.Long-term regular aerobic exercise can reduce the weight,blood fat and blood sugar of rats.3.Aerobic exercise may inhibit the progression of atherosclerosis in rats by mediating arachidonic acid metabolism,unsaturated fatty acid biosynthesis pathway,pyrimidine metabolism and aldosterone regulation of sodium reabsorption pathway.4.Arachidonic acid,cytosine and cortisone can be used as the recovery indicators of aerobic exercise in the intervention of atherosclerotic diseases. |
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