Effect Of Aerobic Exercise On Adiponectin MRNA Expression During The Formation Of Atherosclerosis In ApoE-Deficient Mice

Atherosclerosis (AS) is a multi-pathogenies cardiovascular disease that severely impacts on human health, the latter is the leading cause of morbidity and mortality worldwide. Now, AS is a polygenetic and complex disease with rising prevalence. In developed countries, cardiovascular disease ranks as the top contributing factor for mortality. So AS prevention and treatment has been a principal task to improve human health.Now the main method to prevent and treat AS should be paid to the control of diet, aerobic exercise and the living custom of healthy. Recent reports show that aerobic exercise has been proved to have positive effect on AS, the mechanisms of reduction atherogenic risk of exercise are not completely understood.Purpose: With the extension of scientific research methods, it suggest that adiponectin (APN) of visceral adipose tissue is likely to one of the most important factors to affect cardiovascular. At the same time, aerobic exercise has been proved to have positive effect on AS. So we speculate that aerobic exercise may have positive effect on AS by the change of APN mRNA in adipose tissue. It may be another theoretical for the positive effect on AS by exercise. The purpose of the present experiment is to observe the change of APN mRNA and speculate the effect. It will provide further theoretical evidences for the recognition of AS by exercise.Methods: Twenty 8-week-old Apo-E deficient mice were randomly assigned to two groups: Apo-E deficient mice control group (C); aerobic exercise-trained group (E).The mice were fed by high fat diet. Mice in the aerobic exercise-trained groups were trained for 14 weeks. At the end of experiment, all mice were killed, and the blood, epididymal fat as well as perirenal fat were obtained. Atherosclerotic was observed. APN mRNA in adipose tissue was tested by reverse transcription-polymerase chain reaction (RT-PCR). Serum was test by enzyme-linked immunosorbentassay (ELISA).Results:â‘ There is no evidence change in body weight and the increase of body weight between two groups after 14 weeks aerobic exercise. But epididymal fat as well as perirenal fat of C group was significantly higher than that of E group. So it suggested that 14 weeks aerobic exercise can not change the body weight both of two groups, but epididymal fat as well as perirenal fat of E group were significantly decrease.â‘¡Photograph of the atherosclerotic lesions in aortic cross section were stained with HE and ORO. The vascular of E group was significantly better than C group. It suggested that aerobic exercise significantly decreased the development of atherosclerotic plaque.â‘¢After 14 weeks aerobic exercise, the serum APN had no significantly difference between C group and E group. Aerobic exercise had not affected their blood lipid and normality. It suggested that aerobic exercise can play a role in anti-atherogenesis, which is probably independent in the channel of reducing blood lipid.â‘£The results of RT-PCR showed that, APNmRNA in the adipose tissue of E group was significantly higher than that of the C group (P<0.05); Serum APN of E group was significantly higher than that of the C group (P<0.01) too. It suggested that 14 weeks aerobic exercise can significantly increase APNmRNA in the adipose tissue as well as serum APN.Conclusions:â‘ 14 weeks aerobic exercise can provide the greatest benefit in the prevention of the progression of AS.â‘¡14 weeks aerobic exercise can significantly increase APNmRNA in the adipose tissue as well as serum APN. Aerobic exercise is likely to one of the factor to anti-atherogenesis.