Micro-CT Evaluation Of The Effect Of Different Doses Of Titanium Particles On Implant Osseointegration

Objective The purpose of this study was to establish a maxillary implant model in SD rats and explore implant osseointegration under the action of titanium particles in vivo in order to provide a theoretical basis for the dental implants placed in previously failed sites.Methods Twelve male SD rats were randomly divided into three groups:control group,0.1 mg titanium particles group(L-Ti group),and 1 mg titanium particles group(H-Ti group).Each group included four rats.Bilateral maxillary first molars were extracted in each group.Four weeks after tooth extraction,bilateral implant surgery was performed.In the control group,sterile PBS was placed in the implant holes,and implants were inserted.In the L-Ti group,a 0.1mg titanium particle suspension was placed in the implant holes before implants were inserted.In the H-Ti group,a 1 mg titanium particle suspension was placed in the implant holes before insertion of implants.Four weeks later,the rats were sacrificed by anesthetic overdose,and the maxillary bones were collected for micro-CT analysis.Result 1.Micro-CT 3D reconstruction results showed that four weeks after implantation,a large amount of bone tissues could be seen around the implants in the control group and the L-Ti group,and osseointegration around the implants was good.Meanwhile,the control group was better than the L-Ti group.In the H-Ti group,the amount of new bone tissues around the implant was less,and osseointegration around the implants was poor.2.Quantitative assessments of the new bone formation results showed that the BV/TV,Tb.Th,and Tb.N of the bone around the implant in the region of interest were significantly lower in the H-Ti group than in the L-Ti group and control group.Meanwhile,BS/BV and Tb.Sp were significantly higher in the H-Ti group than in the L-TI group and control group(P < 0.05).Compared to those in the control group,BV/TV,and Tb.N were lower in the L-Ti group,but the difference was not statistically significant(P > 0.05).Conclusions Through experiments on implants in the maxillary bone of rats,it was proven that a high dose of titanium particles could significantly inhibit the formation of new bone around implants and reduce the rate of osseointegration around implants.A low concentration of titanium particles had no significant effect on the formation of new bone around implants and the osseointegration rate.