Investigation Of Influencing Factors Related To Nutritional Short Stature (Spleen Dysfunction) And Microarray Analysis Of Intestinal Flora

Purpose: This topic is divided into two parts:(1)Analysis of the factors influencing nutritional short stature(evidence of spleen dysfunction)through parental questionnaires.(2)By comparing the differences in the types and amounts of intestinal flora between children with short stature(spleen dysfunction)and other short children with normal diet,we summarize and analyze the characteristics of intestinal flora in children with nutritional short stature(spleen dysfunction),in order to provide a basis for clinical diagnosis and treatment.Methods:(1)To collate and analyze 1920 questionnaires collected from children with nutritional short stature in the clinic,and to count the proportion of the category of spleen dysfunction and their related influencing factors in them,and to analyze the importance of spleen dysfunction in children with short stature.(2)Forty cases of children with nutritional short stature with evidence of spleen dysfunction were selected as the observation group,and a questionnaire was filled in before any treatment was given.Forty children with idiopathic short stature were selected as the control group,questionnaires were collected and intestinal flora were tested to compare the differences between the two groups.Results:Part 1:Investigation of influencing factors related to nutritional short stature(spleen dysfunction)A total of 2,895 questionnaires were collected,including 1,920 with evidence of spleen dysfunction.Among the factors influencing the evidence of spleen dysfunction,feeding patterns after birth,the amount of milk added after weaning,the addition of complementary foods,eating habits and dietary preferences,history of previous illnesses,use of antibiotics,and exercise accounted for the majority.Part 2:Comparison of factors affecting children with nutritional short stature(evidence of spleen dysfunction)and children with idiopathic short stature and intestinal flora1.Comparison of baseline data1.1The age,sex,birth mode,feeding mode,height and weight of children in the two groups were compared,P > 0.05,and the differences were not statistically significant and comparable.1.2After weaning from breast milk,the number of children with Spleen Movement Disorders who added milk was less than that of children with idiopathic short stature,and the number of children with idiopathic short stature who chewed coarsely,ate more liquid and semi-liquid diets than that of children with idiopathic short stature,and exercised less than that of children with idiopathic short stature,P < 0.05,the difference was statistically significant.There was no significant difference between the two groups in the comparison of supplementation status,history of previous illness,antibiotic use and exercise duration,P > 0.05.2.Comparison of intestinal flora2.1Comparison of the differences: In both groups,the intestinal flora of children with spleen disorders had 7 fewer species than those of children with normal diets in terms of composition;The dominant phyla(>1%)in both groups were Firmicutes,Proteobacteria,Actinobacteria,Bacteroidetes,Epsilonbacteraeota,and Fusobacteria,and the differences were not statistically significant when comparing the two groups;Seven genera had statistically significant differences when compared at the genus level;The variability in abundance between the two groups was mainly reflected in the strains,and the differences between the two groups at the species level were statistically significant for 32 strains,accounting for the larger differences in Clostridium scindens,Lactobacillus alimentarius,Clostridium aldenense,Brevibacterium halotolerans.2.2Diversity analysis: Alpha-Diversity(α-diversity analysis)P>0.05,the difference between the two groups is not statistically significant(shannon\simpson\sobs\chao\ace);Beta-Diversity(β-diversity analysis)applying principal component analysis(PCA),the results show that the similarity of the composition of the flora between the two groups is high.3.ROC curve: AUC=0.706.The random forest model is considered to have better classification performance and higher confidence level.4.Correlation analysis: Spear-man correlation analysis was performed between the strains that accounted for a larger proportion(>0.1%)and the degree of anorexia,and Clostridium scindens,Lactobacillus alimentarius,Clostridium aldenense and the degree of anorexia were positively correlated,and P < 0.05.The correlation was statistically significant;the correlation between Brevibacterium halotolerans and the degree of anorexia was not statistically significant(P > 0.05).Conclusions:1.Spleen transport deficiencies account for a large proportion of nutritional short stature,and the factors that influence this are mainly feeding patterns after birth,the amount of milk added after weaning from breast milk,the addition of complementary foods,eating habits and dietary preferences,history of previous illnesses,use of antibiotics,and exercise.Compared with children with idiopathic dwarfism,there were statistically significant differences in the types of milk added,eating habits,and the amount of liquid and semi-liquid diet after weaning from breast milk in children with nutritional short stature(evidence of spleen dysfunction).2.The differences in the relative abundance of intestinal flora in children with nutritional short stature with evidence of spleen dysmotility compared to children with idiopathic short stature were mainly at the species level.3.There was no significant difference in the diversity of intestinal flora between the two groups of children.4.The analysis between the species that accounted for a larger proportion(>0.1%)and the level of anorexia showed a positive correlation between Clostridium scindens,Lactobacillus alimentarius,Clostridium aldenense and the level of anorexia.