Study On The Expression Level And Clinical Diagnostic Value Of Serum MiR-145-5p In Patients With Alzheimer’s Disease

ObjectiveTo investigate the expression and clinical diagnostic value of serum miR-145-5p in patients with Alzheimer’s disease(AD).MethodsThirty-six patients with AD who admitted to the Department of Neurology of the Affiliated Hospital of Jiangsu University from June 2020 to December 2020 were enrolled in AD group,and 18 healthy people were selected as the control group.The expression level of miR-145-5p was detected by real-time fluorescence quantitative PCR before and 6 months after Donepezil treatment in AD group and control group,and the expression of Insulin-like Growth Factor 1 Receptor(IGF-1R)was detected by ELISA Kit,the cognitive function of AD patients was evaluated by MMSE and MoCA.The correlation between the expression level of serum miR-145-5p and the scores of MMSE and MOCA and IGF-1R were analyzed.The diagnostic efficacy of miR-145-5p for AD was evaluated by receiver operating characteristic(ROC)curve.ResultsThe expression level of serum miR-145-5p in AD group before treatment was higher than that in control group,and the expression level of serum miR-145-5p after treatment was lower than that before treatment,with statistically significant differences(P<0.05).There was significant negative correlation between expression level of serum miR-145-5p and MMSE,MoCA scores and IGF-1R(r=-0.707,r=-0.687,r=-0.608,P<0.05).ROC curve showed that the area under the curve of serum miR145-5p was 0.773(95%CI:0.619-0.927,P<0.05),the sensitivity was 88.9%,the specificity was 66.7%.ConclusionThe expression of serum miR-145-5p is up-regulated in AD patients,which has a certain application value for AD screening,and is expected to become a potential biomarker for clinical auxiliary diagnosis of AD.