Preliminary Study On The Safety Of Tartary Buckwheat Vinegar Drink

Objective:To study whether Tartary buckwheat vinegar drink has acute and subacute toxicity through acute oral toxicity test and 28-day oral toxicity test,to study the safety of Tartary buckwheat vinegar drink,to provide animal test basis for safe and healthy medicine and food homologous food,to instruct nurses to provide patients with scientific dietary health guidance.Methods:1.Experimental methods(1)Acute oral toxicity test of Tartary buckwheat vinegar: 40 ICR mice of clean grade(male and female half)were randomly divided into dose group and control group.The tested animals of the dose group were given 100 times of the recommended intake of the Tartary buckwheat vinegar drink twice by intragastric administration at a volume of 20 m L/kg BW,and the tested animals of the control group were given distilled water by gavage.After intragastric administration,the mice were observed for 14 days continuously.The general condition observation,weight change,gross anatomy examination results,and visceral coefficient of each group were recorded.(2)28-day oral toxicity test of Tartary buckwheat vinegar: 80 SD rats of clean grade(male and female half)were divided into four groups: high,medium,low dose groups,and a control group randomly.High,medium,and low dose groups were given 100 times,50 times,and 25 times of the recommended intake of the Tartary buckwheat vinegar drink by intragastric administration at a volume of 10 m L/kg BW for 28 days continuously,and the control group was given distilled water by intragastric administration.During the experiment,the general observation,body weight change,food intake,and food utilization rate of each group were recorded.At the end of the experiment,hematology examination,blood biochemical examination,gross anatomy examination,visceral coefficient measurement,and histopathological examination were performed.2.Statistical methodsSPSS28.0 software was used for the statistical processing of the obtained data.The independent sample t-test method was used to analyze the observed indicators of the dose group and the control group in the acute oral toxicity test;One-way analysis of variance(One-Way ANOVA)was used to deal with the changes in body weight,food intake,food utilization,hematological examination results,blood biochemical examination results and organ coefficients of rats.The data were further subjected to post hoc multiple comparisons(Dunnet t-test)to analyze whether there is a statistical difference between each dose group and the control group.All data are expressed as Mean ± Standard Deviation(±SD),and the difference is statistically significant when P<0.05.Results:1.Acute oral toxicity test of Tartary buckwheat vinegar(1)General observation: During the 14 days test,the mental state,autonomic activity,and behavior of mice in each group did not see abnormalities.The mice did not appear symptoms of poisoning,and no death.(2)Body weight change: During the test,the growth and development of male and female mice in the dose group were good,and their body weight was not affected by the tested substance.There was no significant difference in body weight change between the dose and control groups(P>0.05).Within 14 days,the body weights of male and female mice in the dose group increased from 24.07±2.33 g,22.93±2.38 g to 32.95±2.34 g,25.44±2.56 g,respectively;the body weights of male and female mice in the control group increased from 23.90±2.80 g,23.25±2.30 g to 33.06±2.82 g,25.04±2.25 g.(3)Gross anatomy examination and visceral coefficient measurement: At the end of the test,no abnormal changes were found in the major organs such as the hearts,livers,spleens,kidneys,and stomachs of the rats.At the same time,compared with the control group,there was no significant difference in the visceral coefficients of the main organs in the dose groups(P>0.05).2.28-day oral toxicity test of Tartary buckwheat vinegar(1)General observation: During the experiment,the autonomic activity,behavior,and mental state of each rat are normal.The rats did not appear symptoms of poisoning and death.(2)Body weight change: The growth and development of rats in all dose groups were good,and the body weight was not affected by the tested substance.There was no significant difference between the dose and control groups(P>0.05).During the test,the body weights of male and female rats in the high-dose group increased from 83.91±7.97 g,77.47±7.77 g to 338.30±34.26 g,228.53±22.34 g,respectively;the body weights of male and female rats in the middle-dose group increased from 79.59±6.69 g,81.59±4.87 g to338.41±27.72 g,234.67±20.20 g,respectively;the body weights of male and female rats in the low-dose group increased from 78.30±9.32 g,74.92±8.93 g to 346.95±51.12 g,216.42±27.48 g,respectively;the body weights of rats and female rats in the control group increased from 76.65±7.28 g,78.08±5.91 g to 377.79±30.92 g,238.95±21.39 g,respectively.(3)Food intake and food utilization rate: In comparison with the control group,there were no significant differences in food intake and food utilization rate of each dose group(P>0.05).(4)Histopathological examination: Compared with the control group,the WBC of female rats in the low-dose group was higher than that in the control group,with a significant difference(P<0.05),but it was still within the normal range,so it had no clinical significance.There was no significant difference in the hematological indexes between the other dose groups and the control group(P>0.05).(5)Blood biochemical examination: Compared with the control group,the liver function biochemical indexes,renal function and other biochemical indexes of each dose group were not different significantly(P>0.05).(6)Gross anatomical examination and visceral coefficient measurement: At the end of the test,no obvious abnormal changes were found in the major organs such as the hearts,livers,spleens,kidneys,and stomachs of the rats.In the meantime,compared with the control group,the absolute weight of the spleen of male rats in the middle-dose group was lower,with a significant difference(P<0.05),but it was still within the normal range,so it had no clinical significance.There was no significant difference in absolute organ weight and visceral coefficient between the other dose groups and the control group(P>0.05).(7)Pathological examination: The examination results showed that the major organs such as the hearts,livers,spleens,kidneys,and stomachs in the high-dose group were normal,and there was no significant difference compared with the control group.Conclusion:The results of the acute oral toxicity test and the 28-day oral toxicity test of Tartary buckwheat vinegar drink indicate that Tartary buckwheat vinegar drink has no short-term toxic effect and toxic characteristics,and it is preliminarily verified that Tartary buckwheat vinegar drink is safe and non-toxic,and can be promoted as a natural and safe medicine and food homologous food.