Expression And Prognostic Value Of RNase2 In Glioblastoma Multiforme

Objective: Human ribonuclease A(RNase A)family plays an important role in human life activities.It can not only hydrolyze RNA,but also participate in the processes of cell maturation and apoptosis.At the same time,angiogenesis and host defense are also closely related to it.At present,there is little research on its member RNase2.Our aim was to explore the relationship between RNase2 and glioblastoma multiforme based on the Cancer Genome Atlas(TCGA)database.Methods : The clinical and survival information of Glioblastoma Multiforme(GBM)patients were collected from TCGA database.We compared the expression of RNase2 in GBM and normal tissues by Wilcoxon rank sum test,and analyzed the relationship between RNase2 and clinicopathological features by logistic regression.In addition,Kaplan-Meier and Cox regression analysis were used to evaluate the correlation between RNase2 and survival and its effect on radiotherapy and chemotherapy in patients with GBM.At the same time,we used TIMER and GEPIA databases to study the relationship between RNase2 expression and immune cell infiltration and its corresponding biomarkers,GO and KEGG were used to evaluate the biological function of RNase2.The Protein-Protein Interaction Networks of RNase2 was constructed by STRING database.Result:Compared with normal tissues,the expression of RNase2 in GBM tumor tissues was significantly higher(p<0.001).The overall survival rate(p=0.005),disease-specific survival rate(p=0.004)and progression free survival rate p=0.004)of GBM patients with high expression of RNase2 were significantly shorter than those with low expression of RNase2(p=0.028).Patients with low RNase2 expression could not benefit from radiotherapy and chemotherapy(p=0.058).Univariate and multivariate Cox regression analysis showed that Cox expression was independently correlated with OS(univariate p=0.005,multivariate p=0.002).The tumor microenvironment analysis showed that the high expression of RNase2 was accompanied by more infiltration of immune activators such as dendritic cells,B cells and macrophages.At the same time,it was significantly negatively correlated with CD8 + T cells that inhibited tumor growth.Rnase2 is likely to participate in the malignant process of M2 macrophages promoting tumor growth and reduce tumor immune response by inhibiting T cell effect.Correlation analysis showed that RNase2 was significantly positively correlated with immune checkpoints that inhibited tumor immune response.Go,KEGG and PPI network analysis showed that RNase2 was mainly related to immunoglobulin binding,Ig G binding and other functions.It also participates in the immune response processes such as neutrophil activation and phagocytosis,and is related to the biological processes and response pathways such as the regulation of T cell tolerance induction and the interaction between lymphocytes and non lymphocytes.Conclusion: The high expression of RNase2 is related to the disease progression and poor prognosis of GBM,it also relate to the enhancement of immune cell infiltration.It may become a potential biomarker for the diagnosis and prognosis of glioblastoma patients,and it also has the potential to become a therapeutic target of GBM in the treatment site.