| Objective:To explore HMGA2,IMP3 expression in cervical squamous intraepithelial lesions and cervical cancer,and to research their significance in different cervical lesions and their correlation,so as to provide new ideas for cervical cancer early prevention,early diagnosis and optimization of treatment.Methods:According to the inclusion and exclusion criteria,the cervical tissues of patients who underwent surgical treatment in gynecology from January,2022 to December,2022,which were stored in the Pathology Department of Taizhou People’s Hospital,were selected,including normal cervical tissues.There were 30 cases of NCE,32 cases of low-grade cervical squamous intraepithelial lesion(LSIL),38 cases of high-grade cervical squamous intraepithelial lesion(HSIL)and 45 cases of cervical cancer(CC),a total of 145 cases.Immunohistochemical method was used to detect the expression of HMGA2 and IMP3 in all selected cervical tissues.The data were analyzed by SPSS 26.0 statistical software,and the relationship between them and different cervical lesions,the relationship between them and the clinicopathological features of cervical cancer and their correlation were analyzed,so as to explore the significance of them in early prevention,early diagnosis and optimal treatment of cervical cancer.Results:1.HMGA2,IMP3 in normal cervical tissues and different expression of cervical lesionThe positive expression rates of HMGA2 in NCE,LSIL,HSIL and CC were respectively 16.67%(5/30),34.38%(11/32),55.26%(21/38),82.22%(37/45).The positive expression rates of IMP3 in NCE,LSIL,HSIL and CC were respectively 0.00(0/30),3.13%(1/32),18.42%(7/38),66.67%(30/45).HMGA2 and IMP3 expression in NCE,LSIL,HSIL and CC showed a trend of rising and its expression are strict with cervical lesions,The differences were statistically significant.(H=30.927,62.216,P<0.001).2.HMGA2,IMP3 positive expression in the compare between different cervical lesion groupHMGA2 in NCE/HSIL,NCE/CC,LSIL/HSIL,LSIL/CC,HSIL/CC expression differences are statistically significant in the group.(Z=3.556,6.063,2.405,5.347,3.581,P=0.000,0.000,0.016,0.000,0.000).IMP3 in NCE/HSIL,NCE/CC,LSIL/HSIL,LSIL/CC,HSIL/CC expression differences are statistically significant in the group.(Z=2.461,6.345,2.008,6.345,5.700,P=0.014,0.000,0.045,0.000,0.000).HMGA2,IMP3 positive expression in the expression of an NCE/LSIL group differences were not statistically significant.(Z=1.622,0.968,P=0.105,0.333).3.The HMGA2 positive relationship expression and clinicopathological features of cervical cancerHMGA2 postoperative FIGO staging and muscular,differentiation degree,infiltration and lymphatic metastasis,vascular invasion and HPV types correlation,The differences were statistically significant.(χ2=21.019,17.242,8.955,9.791,11.144,9.815,P=0.001,0.004,0.027,0.015,0.010,0.031).HMGA2 with age,pathological type,nerve infiltration and near the palace is not relevant,the differences were not statistically significant.(χ2=0.480,3.175,2.265,3.825,P=0.954,0.914,0.562,0.283).4.The IMP3 positive relationship expression and clinicopathological features of cervical cancerIMP3 and postoperative FIGO stage,pathological type,differentiation degree and lymph node metastasis,vascular invasion,nerve infiltration and HPV types,The differences were statistically significant.(χ2=12.248,11.478,12.928,9.357,9.502,12.001,10.025,P=0.033,0.022,0.027,0.017,0.021,0.005,0.035).IMP3 with age,invasive depth and near the palace is not relevant,the differences were not statistically significant.(χ2=2.041,3.129,1.510,P=0.607,0.378,0.701).5.HMGA2,IMP3 expression in cervical cancer tissue of the correlationIn cervical cancer tissues HMGA2,there is a positive correlation relationship between IMP3 expression.(r=0.520,P<0.001).6.HMGA2,IMP3 regression analysis in cervical lesions(1)Analysis of HMGA2 and whether IMP3 can predict cervical cancer molecular markersThe OR Value Of HMGA2 was 1.382,95% confidence interval(0.82,2.328),P=0.225,the difference was not statistically significant.The results indicate that HMGA2 cannot be used as a molecular marker to predict the occurrence of cervical cancer.The OR value of IMP3 was 4.483,95% confidence interval(2.379,8.4510),P<0.001.The difference was statistically significant,indicating that IMP3 could be used as a molecular marker to predict the occurrence of cervical cancer.(2)Analysis of HMGA2 and whether IMP3 can predict the progression of cervical lesions molecular markersThe results in the predicted LSIL progression to HSIL are shown:The OR value of HMGA2 was 1.695,95% confidence interval(0.859,3.44),P=0.128,the difference was not statistically significant.The OR value of IMP3 was 4.962,95% confidence interval(0.658,33.481),P=0.123,the difference was not statistically significant,indicating that HMGA2 and IMP3 could not be used as molecular markers to predict LSIL progression to HSIL.The results in the predicted HSIL progression to CC are shown:The OR value of HMGA2 was 0.968,95% confidence interval(0.557,1.681),P=0.907,the difference was not statistically significant,indicating that HMGA2 could not be used as a molecular marker to predict HSIL progression to CC.The OR value of IMP3 was 3.172,95% confidence interval(1.667,6.036),P<0.001.The difference was statistically significant,indicating that IMP3 could be used as a molecular marker to predict predicted HSIL progression to CC.Conclusion:1.The positive expression of HMGA2 and IMP3 were positively correlated with the severity of cervical lesions.2.The positive expression of HMGA2 and IMP3 is closely related to the poor prognosis of cervical cancer,which is helpful to evaluate the prognosis of cervical cancer.3.HMGA2 and IMP3 may serve as potential molecular markers to help predict the progression of cervical lesions. |
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