Relationship Between The Expression Of PDK1 、Ki-67 In Epithelial Ovarian Cancer Tissues And The Clinicopathological Features In Patients

Objectives To investigate the expression of PDK1 and Ki-67 in serous ovarian cancer.To analyze the relationship between the expression of the two proteins and the clinicopathological characteristics of patients with serous ovarian cancer.We suppose to provide new ideas for evaluating the prognosis of patients with serous ovarian cancer.Methods 93 patients diagnosised as serous ovarian cancer diagnosed in the Department of Obstetrics and Gynecology,affiliated Hospital of North China University of Technology,from January 2001 to June 2020,were selected as the case group,and 50 patients with ovarian serous cystadenoma at the same time were selected as the control group.We collected clinical data and wax block of patients in two groups.Immunohistochemistry(IHC)was used to detect the expression of PDK1 protein and Ki-67 protein.The independent sample t test andχ~2test were used to compare the expression of PDK1 and Ki-67 in serous ovarian cancer and ovarian serous cystadenoma tissues.Theχ~2test and Logistic regression analysis were used to compare the relationship between PDK1 and Ki-67 expression and the clinicopathological characteristics of patients with serous ovarian cancer.Kaplan-Meier analysis was used for survival analysis of patients with serous ovarian cancer.Kendall’s tau-b correlation analysis was used to evaluate the correlation between the two proteins.Results 1 Comparison of basic data between the case group and the control group shows that there was no significant difference in age,menopause or not between them.2 The results of Immunohistochemical showed that the positive expression of PDK1 and Ki-67was higher in case group than those in the case group.3 The expression levels of PDK1and Ki-67 in SOC group were different in different clinicopathological characteristics with(FIGO stage,tissue differentiation,lymphatic metastasis,ascites formation,ascites with cancer cells,recurrence within 3 years and platinum resistance within 3 years)(P<0.05).4Logistic regression analysis showed that high expression of PDK1 is a risk factor for late FIGO stage,poor tissue differentiation,lymphatic metastasis and ascites positive(P<0.05);the high expression of Ki-67 is a risk factor for late FIGO staging and ascites formation(P<0.05).5 Survival analysis showed:the mean PFS and OS of patients with low expression of PDK1 were 7.435±0.745 years and 8.498±0.624 years;the mean PFS and OS of patients with high expression of PDK1 were 2.130±0.176 years and 3.484±0.298 years;the mean PFS and OS of patients with low expression of Ki-67were 7.598±0.686 years and8.912±0.540 years;the mean PFS and OS of patients with high expression of Ki-67were1.877±0.139 years and 3.191±0.256 years,all difference were statistically significant(P<0.05).6 Kendall’s tau-b correlation analysis showed that the expression levels of PDK1and Ki-67 were significantly correlated(r=0.752,P=0.000).Conclusions 1 The expression levels of PDK1 and Ki-67 in SOC tissue were higher than those in control group.2 The higher expression of PDK1 and Ki-67 is related to the clinicopathological characteristics of patients with SOC.When the expression of PDK1and Ki-67 is high,the risk of patients with adverse prognostic factors is increased,patients are more prone to late FIGO stage,poor tissue differentiation,lymphatic metastasis and ascites positive,and more prone to recurrence and platinum resistance.Furthermore,PFS and OS will significantly shorten.Figure 6;Table 10;Reference 80...