Effect And Mechanism Of Mahonia Bealei(Fort.)Carr.on Acute UC In Mice Induced By DSS Based On NF-κB Inflammatory Signal Pathway

Objective: To observe the effect of Mahonia bealei(Fort.)Carr.on colonic inflammation in UC mice and explore its mechanism from the NF-κB signaling pathway.Methods: Forty-eight C57BL/6J mice were randomly divided into normal group,model group,Mahonia bealei(Fort.)Carr.low-dose group(2.3g/kg),medium-dose group(4.6g/kg),high-dose group(9.2g/kg)and mesalazine group(0.2g/kg),with 8 mice in each group.The normal group drank water freely every day,and the other groups drank 2.5% dextran sulfate sodium(DSS)freely.UC mouse model was established,and the modeling and gavage were carried out simultaneously for 10 consecutive days.During the period,body weight changes and disease activity index(DAI)scores of mice were recorded daily.After the experiment,the mice were sacrificed by cervical dislocation.The length of the colon was observed and recorded.The pathological state of the colon tissue was observed by hematoxylineosin(HE)staining.The levels of tight junction proteins Claudin-1,Occludin and ZO-1 in the colon tissue were detected by Western blotting(WB).The levels of inflammatory factors TNF-α,IL-10,IL-1β and IL-6 in the colon tissue were detected by enzyme-linked immunosorbent assay(ELISA).The level of NF-κB protein in cytoplasm and nucleus of colon tissue was detected by Western blotting.Results: Compared with the normal group,the body weight of mice in the model group was significantly decreased,the disease activity index(DAI)score was significantly increased,the colon length was significantly shortened(P<0.01),the pathological damage of colon tissue was significant,the pathological score was significantly increased(P<0.01),the levels of tight junction proteins Claudin-1,Occludin and ZO-1 in colon tissue were significantly decreased(P<0.01),the levels of pro-inflammatory factors IL-1β,IL-6 and TNF-α in colon tissue were significantly increased,the level of antiinflammatory factor IL-10 was significantly decreased(P<0.01),the level of NF-κB protein in the cytoplasm of colon tissue was significantly decreased,and the level of NF-κB protein in the nucleus was significantly increased(P<0.01).Compared with the model group,the body weight of the mice in the Mahonia bealei(Fort.)Carr.medium-dose and high-dose group and the mesalazine group was significantly increased,the DAI score was significantly decreased,the colon length was significantly increased(P<0.05,P<0.01),the pathological damage of the colon was significantly improved,the pathological score of the medium-dose and high-dose group was significantly decreased(P<0.05,P<0.01),and the protein levels of tight junction proteins ZO-1,Occludin and Claudin-1 in the colon tissue of the Mahonia bealei(Fort.)Carr.medium-dose and high-dose group were significantly increased(P<0.05,P<0.01),the levels of pro-inflammatory factors TNF-α,IL-1β and IL-6 in colon tissue were significantly decreased,the level of anti-inflammatory factor IL-10 was significantly increased(P<0.05,P<0.01),the level of NF-κB protein in the cytoplasm in the colon tissue of the Mahonia bealei(Fort.)Carr.medium-dose and high-dose group was significantly increased,and the level of NF-κB protein in the nucleus was significantly decreased(P<0.05,P<0.01).Conclusion: The experimental results show that Mahonia bealei(Fort.)Carr.can improve the inflammatory response of colon tissue in UC mice induced by DSS,improve the function of colonic epithelial mucosal barrier in mice,maintain the integrity of tight junctions,and protect the intestinal mucosal.The mechanism may be related to the regulation of NF-κB signaling pathway.