| Objective:In clinical practice,dental abnormalities and edentulism are widespread and have an impact on the patient’s chewing and aesthetics.Fixed prostheses are frequently utilized in the treatment of dental abnormalities and edentulism because they are both aesthetically pleasing and comfortable.Plaque biofilm will adhere to the tooth surface and restorative material surface in the oral cavity,causing not only the pathogenesis of caries and periodontal disease,but also secondary caries and restorative gingivitis,resulting in clinical treatment failure and a reduction in prosthesis service life.The purpose of this study is to investigate the microbial composition of the supragingival plaque biofilm on the surface of fixed prostheses,as well as the differences between it and the microbial composition of natural supragingival plaque,in order to guide patients in selecting fixed prostheses with appropriate materials,paying attention to oral hygiene habits,reducing the risk of periodontal diseases,improving the success rate of fixed prostheses,and extending the lifespan of fixed prostheses.Method:Patients undergoing full-crown prosthesis treatment at Jilin University Dental Hospital were chosen as the experimental group,and the patients were required to have healthy periodontium and a natural tooth with the same name on the opposite side of the patient’s tooth for full-crown prosthesis.Twenty participants were randomly assigned to one of two groups: cobalt-chromium metal porcelain crowns and zirconium oxide all-ceramic crowns.Furthermore,patients who were periodontally healthy and had never had full-crown prostheses were chosen as the control group,with 20 individuals included.This study involved 40 participants,and60 supragingival plaque samples were taken.All samples’ DNA was sequenced using16 S rDNA high-throughput sequencing technology,and the sequencing results were bioinformatically processed using Qiime software,R software,and PICRUSt2 software.Results:The V3-V4 high variation region of the 16 S rDNA gene was sequenced using high-throughput sequencing technologies.From 60 samples,3857306 high-quality sequences were retrieved,and 3221 ASVs were clustered based on 100% sequence similarity.There were 26 phyla and 284 genera identified.Among them,the major phyla Actinobacteriota,Proteobacteria,Firmicutes,Bacteroidota,Fusobacteriota,Campylobacterota,Patescibacteria,Bacteroidetes,Spirochaetota,Synergistota,Actinobacteria,Candidatus＿Saccharibacteria,Desulfobacterota(relative abundance >1%).Major genera Corynebacterium,Capnocytophaga,Actinomyces,Neisseria,Leptotrichia,Streptococcus,Rothia,Fusobacterium,Veillonella,Haemophilus(relative abundance >5%).The microbial composition and structural diversity of supragingival plaque on the surface of fixed prostheses differed from that of normal natural supragingival plaque in this study,and the content of periodontally related pathogens,such as g＿Treponema,g＿Selenomonas,g＿Porphyromonas,and g＿Prevotella＿7,were relatively high.Fixed prostheses revealed functional expectations in the body group,microbial metabolism was relatively active.Lipopolysaccharide biosynthesis,clycine,serine,and tnreonine metabolism,all of which are related with the development of periodontal disease,were relatively high.The composition and structure of the microflora on the surface of zirconia all-ceramic crowns are more similar to that of natural teeth’s microbiota than that of cobalt-chromium metal porcelain crowns.Conclusion:The gingival plaque on the surface of fixed prostheses has a different microbial community than natural teeth,and the presence of the prosthesis increases the risk of periodontal disease in the abutment teeth,whereas zirconia all-ceramic crowns are less risky than cobalt-chromium metal crowns.To limit the risk of periodontal disease,all-ceramic prostheses are advised clinically to reduce periodontal responses in abutment teeth,and doctors should provide adequate oral hygiene instruction. |
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