| Objective:The purpose of this study is to investigate the expression of C10orf10 gene in glioma cells under hypoxia environment and its effect on the growth of glioma cells and the prognosis of glioma patients.Methods:1.Data sets related to glioma and hypoxia were screened from Gene Expression Omnibus(GEO)database,and C10orf10 was screened out from glioma cell lines treated with hypoxia by q PCR and Western blot through bioinformatics analysis.2.Download The Cancer Genome Atlas(TCGA)and The Glioma sample dataset from The Chinese Glioma Genome Atlas(CGGA),and analyzed the impact of the C10orf10 expressed on the prognosis of glioma.3.The tumor tissue samples and follow-up data of 68 patients with glioma who underwent surgical treatment in Xiangya Hospital of Central South University from March 2017 to January 2021 were collected.The expression of C10orf10 in glioma samples was detected by RT-PCR,and the relationship between C10orf10 and prognosis was analyzed by Kaplan-Meier method.4.By constructing glioma cell lines that interfere with C10orf10expression,CCK8 and plate clonogenesis assay were used to evaluate the effect of C10orf10 on glioma cell proliferation.Results:1.The expression of C10orf10 gene in glioma under hypoxia was up-regulated.QPCR and WB results showed that the expression of C10orf10 gene in glioma cell lines treated with hypoxia was higher than that in normoxia group.2.The expression of C10orf10 in glioma samples in TCGA and CGGA database increased with the increase of WHO grade.Survival analysis results in TCGA database showed that the mean OS of C10orf10high expression group was 22.92 months,and the mean OS of C10orf10low expression group was 32.84 months.In the CGGA database,the mean OS of C10orf10 high expression group was 30.43 months,and that of C10orf10 low expression group was 51.29 months.In addition,OS of C10orf10 group with high expression level was higher than that of C10orf10 group with low expression level.X~2test was used to analyze the relationship between C10orf10 and some common clinicopathological information in 413 cases of glioma in CGGA database.The results showed that C10orf10 was significantly correlated with age,WHO grade,IDH mutation and co-deletion of 1p/19q chromosome(P<0.05).In the CGGA database,the expression level of C10orf10 in recurrent glioma was higher than that in primary glioma,and the mean OS of patients treated with TMZ was 30.58 months in C10orf10 high expression group,and 48.31 months in C10orf10 low expression group.3.Rt-PCR detection results of tumor tissue samples from 68 glioma patients showed that the expression of C10orf10 increased with the increase of WHO grade.Survival analysis results showed that the mean OS of C10orf10 high expression group was 10.38 months,and that of C10orf10 low expression group was 10.68 months.The OS values of the two groups were statistically different(P<0.05).4.Knockdown C10orf10 expression of glioma cell line CCK8 and cell cloning results showed that the proliferation activity was lower than the control group.Conclusion:1.The m RNA and protein expression levels of C10orf10 in glioma cells increased under hypoxia.2.The expression of C10orf10 in glioma increased with the increase of WHO grade of glioma.3.Patients with higher C10orf10 expression had shorter survival.4.The expression of C10orf10 is higher in recurrent gliomas than in primary gliomas,and in patients treated with TMZ,the higher the expression of C10orf10,the shorter the survival.5.Knockdown of C10orf10 can significantly inhibit the proliferation and clonal formation of glioma cells... |
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