Targeted Capture Of T-bet~+ CDC2s By Functionalized Carbon Nanotubes Promotes The Repair Of Myocardial Infarction

Objective:（1）To investigate the quantity and distribution of T-bet+c DC2A in infarcted tissue and its role in myocardial repair.（2）To construct a functionalized carbon nanotube（CNT-CR4-C4A）^CR2 that moved to the infarct area based on the local microenvironment after myocardial infarction.To explore the therapeutic effect of functionalized carbon nanotubes on myocardial infarction（MI）by analyzing their distribution in vivo and evaluating their effect on T-bet+c DC2A recruitment and retention.Methods:（1）Myocardial infarction tissues were taken for immunofluorescence and DAPI staining,and c DC2s subtypes were analyzed by fluorescence microscopy and flow cytometry.（2）c DC2A was injected into myocardium in situ to treat myocardial infarction.Functional carbon nanotubes were designed and constructed,and injected into rat tail vein to treat myocardial infarction in rats.The distribution in vivo and the recruitment effect of c DC2A were analyzed,and the therapeutic effect was statistically analyzed.Results:（1）T-bet+c DC2A is the main subtype of c DC2s in myocardial infarction tissue,which is mainly distributed in the marginal area of myocardial infarction tissue.T-bet+c DC2A can reduce the infarct size and reduce the degree of myocardial fibrosis,but in situ myocardial injection can not improve the survival rate of myocardial infarction model rats.（3）The functionalized carbon nanotubes injected through the rat tail vein can move to the myocardial infarction area and retain T-bet+c DC2A for long-term recruitment,thus reducing the infarct area,reducing the degree of myocardial fibrosis,and improving the survival rate of rats with myocardial infarction.Conclusions:T-bet+c DC2A is the subgroup of c DC2s cells that promote myocardial repair,mainly distributed in the marginal area of myocardial infarction tissue.T-bet+c DC2A by in situ myocardial injection can reduce infarct size and myocardial fibrosis degree,but can not improve the survival rate of rats with myocardial infarction.The functionalized carbon nanotubes injected through rat tail vein showed the ability to target infarct areas.In the experimental treatment of AMI rats,the functionalized carbon nanotubes recruited T-bet+c DC2A to converge to the edge area of myocardial infarction,thereby reducing the area of myocardial infarction,reducing ventricular remodeling and promoting myocardial repair.In conclusion,functionalized carbon nanotubes（CNT-CR4-C4A）^CR2 is expected to be a new treatment method for AMI.