Differential Expression Of LncRNA In Patients With Traumatic Hemorrhagic Shock Complicated With Acute Traumatic Coagulation And Its Clinical Significance

Objective:The purpose of this study is to screen out the differentially expressed genes of lncRNA which are associated with Acute traumatic coagulation by detecting the gene level changes of lncRNA in the disease progression of traumatic hemorrhagic shock combined with ATC.It provides new potential biomarkers and therapeutic targets for the diagnosis and treatment of ATC.Methods: 4 patients with traumatic hemorrhagic shock complicated with acute traumatic coagulation were selected from the emergency surgery Department of the First Affiliated Hospital of Bengbu Medical College.At the same time,4 healthy people who received physical examination in the First Affiliated Hospital of Bengbu Medical College were selected as controls.The clinical groups were divided into shock group and control group.Peripheral blood samples from each group were centrifuged to obtain human peripheral lymphocytes.The obtained human peripheral lymphocytes were processed to extract RNA.The extracted total RNA was used to remove ribosome r RNA using the MGIEasy r RNA removal kit.After purification,the RNA is fragmented under certain temperature and ionic conditions.A strand of c DNA was then synthesized using random primers and reverse transcriptase in the MGIEasy RNA Directional Library preparation kit,and a double-stranded c DNA was then synthesized using DNA polymerase I and ribonuclease.During c DNA two-strand synthesis,the RNA template is removed and d TTP is replaced by d UTP.The involvement of d UTP prevents the c DNA’s two strands from being amplified in subsequent processes because the polymerase cannot cross the d UTP site on the template when extended.Through PCR of the generated DNA fragment,a single strand DNA fragment was obtained,and then a primer was used to cyclify the single strand DNA to obtain the single strand circular DNA,which was then sequenced on the computer.Low quality,contaminated r RNA and r RNA containing large numbers of unknown bases were eliminated.The result was clean reads.Protein interaction network(PPI)was constructed by quantitative analysis,difference analysis,enrichment analysis and protein interaction study.Results: A total of 245 m RNA and 36 lncRNA were differentially expressed.These differentially expressed genes are enriched in cell growth and death,signal transduction,signal molecule interaction,replication and repair,coagulation factor deficiency,chemokine production,regulation of chronic inflammatory response,apoptotic cell phagocytosis,NOD-like receptor signaling pathway,IL-17 signaling pathway,complement and coagulation cascade.These networks could play an important regulatory role.A total of 281 lncRNA and m RNA differential genes were imported into the STRING database to search the potential interactions between encoded proteins,and a m RNA-lncRNA-mediated protein interaction network(PPI)was constructed.It was found that PPARG,TLR2,ANXA2,CAV3 may interact with other proteins.Conclusion: lncRNA showed significant differential changes in the pathogenesis of ATC.Meanwhile,different genes PPARG,TLR2,ANXA2 and CAV3 that may be associated with the pathogenesis of ATC were screened out,which may provide new potential biomarker and therapeutic target for the diagnosis and treatment of traumatic hemorrhagic shock complicated with ATC.