Changes Of NF-kB Signaling Pathway In Plasma Of Children With Kawasaki Disease And Its Clinical Significance

Objective: To explore the clinical significance of NF-k B signaling pathway in the pathogenesis of Kawasaki disease(KD)by detecting the changes in the levels of Nf-Kb p65,its inhibitor,and its regulatory downstream cytokines in peripheral blood,the severity of coronary artery injury,and their relationship.Methods: A total of 60 children with KD(35 males and 25 females)who were hospitalized in the Pediatric ward of Yijishan Hospital of Wannan Medical College from March 2021 to December 2022 were enrolled.According to the results of echocardiography,the KD children were divided into coronary artery injury group(CAL group,n=15)and no coronary artery injury group(n CAL group,n=45).At the same time,20 cases of fever and pneumonia hospitalized in the same period and 20 cases of healthy hospitalized children who were scheduled for elective surgery were selected as the control group.Children in the fever control group had only recent pulmonary infection,while children in the healthy control group had no recent infection,and heart disease and other serious underlying diseases were excluded by echocardiography.Enzyme-linked immunosorbent assay(ELISA)was used to measure the concentrations of NF-k B65,IkB-α,TNF-α,IL-1β,IL-6,and ET-1,and nitrate reductase method was used to measure the concentration of NO at each time point and each group.To explore the value of NF-k B signaling pathway in the pathogenesis of coronary artery injury by studying the relationship between these indicators.Results:1.The serum levels of NF-k Bp65,IkB-α,TNF-α,IL-1β,IL-6,ET-1 and NO in the KD group were significantly higher than those in the fever control group and the healthy control group before treatment(P < 0.05).2.The serum levels of NF-k Bp65,IkB-α,TNF-α,IL-1β,IL-6,ET-1 and NO in the KD group before treatment were higher than those in the KD group after treatment(P <0.05).3.After treatment,the serum levels of NF-k Bp65 and IL-6 in the KD group were lower than those in the fever control group,but higher than those in the healthy group(P< 0.05).The serum levels of IL-1β and NO in the KD group were higher than those in the fever control group and the healthy control group(P < 0.05).However,the concentrations of IkB-α,TNF-α and ET-1 after treatment were not significantly different from those in the fever group(P > 0.05),but were still higher than those in the healthy control group(P < 0.05).4.The concentrations of NF-k Bp65,IkB-α,TNF-α,IL-1β,IL-6,ET-1 and NO in the CAL group were significantly higher than those in the n CAL group before treatment(P< 0.05).5.Serum NF-k Bp65 and IkB-α(r=0.771,P < 0.05),TNF-α(r=0.755,P < 0.05),IL-1β(r=0.818,P < 0.05),IL-6(r=0.738,P < 0.05)in KD children before treatment.P < 0.05),ET-1(r=0.801,P < 0.05)and NO(r=0.712,P < 0.05).Conclusions:1.NF-k Bp65,IkB-α,TNF-α,IL-1β,IL-6,ET-1 and NO were involved in the pathogenesis of coronary artery endothelial injury.2.Activation of NF-k B signaling pathway may be involved in the pathogenesis of coronary artery endothelial injury in Kawasaki disease.