Study On The Vascular Endothelial Growth Factor And Endostatin In Children With Kawasaki Disease

ObjectiveTrough testing the serum levels of vascular endothelial growth factor and endostatin inchildren with Kawasaki disease, to explore their role in Kawasaki disease，especially Kawasakidisease with coronary artery lesion complication, to provide clinical basis for the study of Kawasakidisease pathogenesis and provide a new reference for the clinical diagnosis and prognosis of Kawasaki disease.Method35 children who had a treatment at Children’s Hospital of Shanxi Province with Kawasakidisease were selected as a research object. and then, according to whether the coronary injury ornot into the coronary injury (CAL) group (n = 13) and without coronary injury (NCAL)group (n = 22)cases. This 35 children collected venous blood in acute and sub acute phase. 20healthy children were collected in Shanxi Children’s Hospital Child health clinic as a controlgroup, and their venous blood was collected. Blood serum was placed in the ultra-lowtemperature refrigerator cryopreservation. When the specimens were all collected, vascularendothelial growth factor and endostatin were measured by enzyme-linked immunosorbentassay (ELISA). Through the heart of color Doppler ultrasound in children with Kawasakidisease group to detect coronary artery whether the injury and determine its inner diameter.Result1. Concentration of VEGF: the concentration of VEGF in the acute phase of Kawasakidisease group (before treatment) was higher than the healthy group (P <0.05), it in sub acute(treatment) decreased significantly than in the acute phase (P <0.05), and is still significantlyhigher than the healthy group (P <0.05). ES concentration: the concentration of ES in the acutephase of Kawasaki disease group (before treatment) was higher than the healthy group (P <0.05),it in sub acute (treatment) decreased significantly than in the acute phase(P <0.05), and is stillsignificantly higher than the healthy group (P <0.05). VEGF / ES ratio: the concentration ofVEGF / ES ratio in the acute phase of Kawasaki disease group (before treatment) was higherthan the healthy group (P <0.05), it in subacute (treatment) decreased significantly than in theacute phase(P <0.05), and is still significantly higher than the healthy group (P <0.05).2. the concentration of VEGF in acute phase of Kawasaki disease in coronary injury groupwere higher than it in without coronary artery injury group, the difference was statistically significant (t = 3.777, P <0.001); ES concentration in acute phase of Kawasaki disease incoronary injury group were higher than it in without coronary artery injury group, the differencewas statistically significant (t = 2.587, P = 0.014); VEGF / ES ratio in acute phase of Kawasakidisease in coronary injury group were higher than it in without coronary artery injury group, thedifference was statistically significant (t = 2.489, P <0.018)3. The concentration of VEGF showed a positive correlation with inner diameter ofcoronary artery (r=0.796, P <0.001), ES concentration showed a positive correlation with innerdiameter of coronary artery (r=0.662, P<0.001), VEGF/ES showed a positive correlation withinner diameter of coronary artery (r=0.775, P <0.001).4. The concentration of VEGF showed a positive correlation with ES (r=0.887, P <0.001).ConclusionVEGF and ES were increased significantly in the acute phase of Kawasaki disease group.And they decreased significantly after treatment in the sub acute stage, suggesting that levels ofVEGF and ES can be used as the observation of the development of the disease process andtreatment indicators, and were significantly correlated with coronary artery dilatation inKawasaki disease. And combined coronary artery injury in the process plays an important role.VEGF / ES ratio increased, showed that the imbalance between VEGF and ES, reflectingKawasaki disease exists endothelial dysfunction. And in predicting coronary injury it have acertain value.