Correlation Between PRDX2 And Spermatogenesis And Sperm Maturation Under Oxidative Stress

Objective:At present,12.5-15% of the couples of childbearing age in China suffer from infertility and infertility according to statistics.The total number of patients has exceeded 40 million.About half of male infertility is caused by male factors.Male infertility is usually accompanied by decreased sperm quality and vitality.Now,alcohol,smoking,drug abuse,poor diet,environmental pollution(such as pesticides)are the main factors causing male infertility.With the change of social diet structure and the proportion of obese people increases,obesity caused by excessive accumulation of reactive oxygen species leads to systemic inflammatory diseases,which is considered to be closely related to male infertility.Among them,peroxiredoxin 2(PRDX2)is a member of the peroxidase family.This enzyme can maintain the homeostasis of the intracellular environment and regulate cell proliferation and apoptosis by clearing excess intracellular reactive oxygen species.It is reported that PRDX2 is closely related to sperm function.However,it is unclear whether PRDX2 is related to the abnormal oxidative stress of spermatogenesis and mature microenvironment.Therefore,we studied the expression of PRDX2 in the testis and epididymis of mice under oxidative stress,and further collected semen samples from patients with obesity and asthenospermia to detect the expression of PRDX2.This paper lays a certain foundation for further exploration of spermatogenesis and maturation function of PRDX2 and oxidative stress.Methods:1.In vitro study: a high-fat diet-induced obese mouse model was constructed,and the mRNA and protein levels of PRDX2 in the testes and epididymis of obese mice and normal mice were detected by RT-qPCR and Western blot;Immunoenzymatic technique and immunofluorescence were used to detect the localization and expression changes of PRDX2 in the testes and epididymis of the two groups of mice.2.In vivo research: construct an oxidative stress model of Sertoli cells and epididymal epithelial cells,and use immunofluorescence to detect the difference in localization and expression of PRDX2;RT-qPCR and Western blot were used to detect the level of mRNA and protein in cells of PRDX2.3.By collecting human semen samples,the semen samples were divided into normal group and obese and weak sperm group according to BMI and sperm motility.Immunofluorescence was used to detect the localization and expression changes of PRDX2 in the two groups of sperm.SPSS was used to analyze the association between PRDX2 and obesity and sperm motility.Results:1.RT-qPCR and Western blot results showed that the expression of PRDX2 in testis and epididymis of obese mice and normal mice decreased.and localized in testicular support cells and epididymal epithelial cells.2.In the oxidative stress model of testicle support cells,excessive addition of hydrogen peroxide led to a decrease in the expression of PRDX2.Excessive addition of cholesterol increases the expression of mRNA and protein levels of PRDX2.Excessive addition of lipopolysaccharides,hydrogen peroxide and cholesterol to epididymal epithelial cells reduced the expression of mRNA and protein levels of PRDX2.3.The immunofluorescence results showed that PRDX2 was mainly localized to the head-to-tail connection of human sperm,and the fluorescence expression was reduced in overweight and weak sperm.The fluorescence expression of PRDX2 was negatively correlated with BMI(r=-0.857,P=0.002)and positively correlated with the percentage of forward motility sperm(r=0.827,P=0.003).Conclusion:The difference in the expression of PRDX2 in vitro and in vitro oxidative stress and the decrease in the expression of overweight and weak sperm indicate that PRDX2 has a certain correlation with spermatogenesis and maturation under oxidative stress.It is presumed to have a protective effect on spermatogenesis and maturation.To provide new research ideas for exploring the weak sperm caused by obesity.