| Background and Purpose: Pancreatic cancer is a highly malignant gastrointestinal tumor with a poor prognosis,Atypical clinical symptoms and early metastasis are the main reasons for its poor prognosis.Long non-coding RNA(LncRNA)is a non-coding RNA that is greater than 200 bases in length and participates in the regulation of cell growth and development,including the process of cancer cell genesis,development,and metastasis.The mechanism of action of LncRNA molecules in pancreatic cancer needs to be further elucidated,therefore,the present study aimed to investigate the expression of LINC00511 in pancreatic cancer and its molecular mechanism of action.Methods: Gene expression of LINC00511 in pancreatic cancer tissues and normal pancreatic tissues was determined by quantitative real-time PCR(qRT-PCR).The association between different expression levels of LINC00511 in pancreatic cancer tissues and the survival of patients was evaluated by Kaplan-Meier survival analysis.Additionally,LINC00511 overexpression and knockdown cells were obtained by lentivirus infection and siRNA interference assay,respectively,to investigate the proliferation,migration,and apoptosis of pancreatic cancer cells in vitro.The tumorigenic activity of LINC00511 overexpression was confirmed by subcutaneous tumorigenic assay in nude mice.The target of LINC00511,miR-195-5p,and its target protein,HMGA1,were obtained by bioinformatics analysis,and their linkage was verified by dual luciferase reporter gene assay.The pro-carcinogenic role of LINC00511 in pancreatic cancer through miR-195-5p/HMGA1 axis was validated by phenotype rescue assay,and the effect of LINC00511 on the corresponding pathway was investigated by Western blot assay.Results: LINC00511 expression was upregulated in pancreatic cancer tissues compared with their corresponding adjacent tissues(p<0.001),and high LINC00511 expression levels were associated with shorter survival cycles in pancreatic cancer patients.In vitro and in vivo experiments demonstrated that LINC00511 significantly promoted the proliferation and tumorigenic capacity of pancreatic cancer cells,and LINC00511 overexpression induced malignant biological behavior of pancreatic cancer cells.Dual luciferase assay and phenotype rescue assay validated that LINC00511 exerted pro-cancer effects through miR-195-5p/HMGA1 axis.Subcutaneous tumorigenesis assay in nude mice confirmed that overexpression of LINC00511 promoted the expression of HMGA1 and the growth of subcutaneous tumors.Conclusion:LINC00511 is a potential molecular indicator for the diagnosis and prognosis of pancreatic cancer.LINC00511 positively regulates HMGA1 expression by binding miR-195-5p,promoting the proliferation and migration of pancreatic cancer cells,inhibiting their apoptosis,and promoting the malignant biological progression of pancreatic cancer. |
PDF Full Text Request