Research On The Medication Rules Of Professor Zhang Mingxue In The Treatment Of Stable Angina Pectoris Of Coronary Heart Diseas

Purpose : Based on data mining technology to summarize the prescription rules of Professor Zhang Mingxue in the treatment of stable angina pectoris of coronary heart disease,summarize the clinical experience of the tutor in the treatment of this disease,and use network pharmacology to reveal the potential mechanism of the core drugs in the treatment of coronary heart disease.Chinese medicine treatment of angina pectoris provides the basis.Materials and Methods:1.Collect patients with complete prescriptions who were diagnosed with stable angina pectoris by Professor Zhang Mingxue from September 2019 to July 2021 in the outpatient department of the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine.According to the inclusion and exclusion criteria,a total of 211 cases that met the criteria were screened,and the relevant data(medical record number,name,gender,age,symptoms,prescription information)were entered into Excel 2019,and a patient information database was established.The frequency statistics of five flavors,meridians,and syndromic elements.And use Modeler 18.0 software to mine the association rules between medicines;cluster the high-frequency medicines in prescriptions by cluster analysis in SPSS 25.0.2.Screen the active ingredients and action targets of the core drugs,obtain the disease targets of coronary heart disease stable angina pectoris,and map the two to obtain the core drug action targets;use Cytoscape 3.7.2 to construct a "core drug-active ingredient-Target”network,construct protein interaction network through String database,use DAVID 6.8database to carry out GO function and KEGG pathway enrichment analysis,explore the possible biological functions and pathways of potential targets,and finally obtain the key to the treatment of coronary heart disease with core compounds Active ingredients and potential mechanism of action.Results:1.The two syndrome elements of qi deficiency(96.6%)and phlegm turbidity(92.9%)are the most common in patients with coronary heart disease and stable angina pectoris treated by Professor Zhang Mingxue,followed by qi stagnation(63.0%),heat accumulation(45.5%),blood Stasis(41.2%).1.The total frequency of drug use in all prescriptions was 3395 times,and a total of 161 drugs were used.Among them,the top 10 traditional Chinese medicines in the frequency of use are astragalus,American ginseng,Pinellia,Gualou,Xiebai,Bupleurum,Cimicifuga,Platycodon grandiflorum,Poria,and keel;The most frequent occurrence of the four qi is temperature,followed by cold,flat,cool,and hot;the most frequent occurrence of the five flavors is sweet,followed by bitter,acrid,light,astringent,sour,and salty;the most frequent occurrence of the meridian is the lung meridian,followed by the spleen,stomach,heart,liver,and kidney.Commonly used drugs are qi-tonifying drugs,phlegm-resolving drugs,qi-regulating drugs,external-relieving(yang)drugs,heat-clearing drugs,and blood-activating drugs.In the analysis of association rules,the drug pairs with high support are "Astragalus-Xiebai","Astragalus-Platinum","Platinum-Chomarin","Bupleurum-Choshma","Astragalus-Choshma","Gualou"-Xiebai,Astragalus","Melon-Astragalus,Xiebai","Xibai-French Pinellia,Gualou","Xiebai-French Pinellia,Astragalus","Melon-American Ginseng,French Pinellia" etc.Cluster analysis was carried out on the top 17 drugs of use frequency,and they were aggregated into 5categories.Category 1: Astragalus,American ginseng,Pinellia,Gualou,Xiebai;Category 2:Cimicifuga,Bupleurum,Platycodon grandiflorum;Category 3: Corydalis,Turmeric,Turmeric,Pueraria;Class 4: Red Peony,Poria,Raw Keel,Raw Licorice;Class 5: Prunella,Cassia.3.The main active ingredients of the core compound are quercetin,kaempferol,naringenin,formononetin,isorhamnetin,β-sitosterol,baicalein,etc.There are 98 intersection targets of drugs and diseases,mainly including JUN、MAPK3、MAPK14、ESR1、MAPK1,etc.The GO enrichment analysis of the core compound shows that the biological process pathways involved mainly include: response to toxic substances,response to hypoxia,signal transduction,positive regulation of gene expression,and negative regulation of apoptosis process.The component expression process involves plasma membrane,extracellular space,nucleus,cytoplasm,extracellular region,etc.The molecular functions involved mainly include: protein binding,enzyme binding,identical protein binding,protein homodimerization activity,zinc ion binding,transcription factor binding,etc.The KEGG pathway enrichment analysis showed that the PI3K-Akt and MAPK signaling pathways were significantly more significant and enriched.Conclusion:1.Professor Zhang Mingxue believes that the pathogenesis of this disease is mainly qi deficiency and phlegm turbidity,and the commonly used treatment method is to nourish qi and.resolve.phlegm.Clinically,"Astragalus-American ginseng-French pinellia-gualou-Xiebai" is often used as the basic formula to treat stable angina pectoris of coronary heart disease.2.Based on network pharmacology research,the main active ingredients in the core compound,such as quercetin,kaempferol,naringenin,and formononetin,can affect JUN、MAPK3、MAPK14、ESR1、MAPK1 through PI3K-Akt and MAPK signaling pathways.Targets such as inhibiting cardiomyocyte apoptosis and protecting vascular endothelium can prevent and delay the formation and development of atherosclerosis.