| BackgroundEndometrial cancer refers to a type of malignant tumor that occurs in the endometrial tissue,with endometrioid adenocarcinoma being the most common,accounting for about 80-90%of all pathological types.In recent years,its incidence has gradually increased and the age of onset has gradually decreased due to factors such as changes in lifestyle habits and the application of hormonal drugs.This trend seriously hinders the increase of life expectancy and quality of life of women,and also adversely affects their reproductive ability.Therefore,endometrial cancer has gradually become a hot research topic in the field of gynecology,but the mechanism of the disease is complex and there are many risk factors,so the research progress is slow.In recent years.more and more studies have demonstrated that the development and metastasis of endometrial cancer may be related to the abnormal expression of some proteins and their alterations caused by the abnormal expression of upstream and downstream signaling pathways.CNTN-1 is considered to be a cell adhesion molecule,and previous studies have suggested that it is associated with neurological development,while its abnormal expression is highly correlated with organic neurological lesions and psychiatric disorders.Several studies in recent years have found that CNTN-1 protein is involved in tumor formation,plays an important role in tumor progression and distant metastasis,is involved in the development of drug resistance in tumor cells,and is closely related to the clinical prognosis of patients.Some of the current studies explain the important role played by CNTN-1 protein in cancer development,infiltration and metastasis.However,very few existing studies have been conducted in the field of gynecology,and there is a lack of studies related to the expression level of CNTN-1 protein in endometrial lesions of patients with endometrial cancer,as well as a lack of deeper exploration of-this protein in the field of endometrial cancer.Objective1.To investigate the expression of CNTN-1 in endometrial cancer normal endometrial tissue:2.To investigate the relationship between the expression difference of CNTN-1 protein and the clinical and pathological characteristics of patients with endometrial carcinoma.MethodsThe expression of CNTN-1 protein in 79 endometrial cancer lesions and 24 normal endometrial tissues was detected by immunohistochemical staining.Twelve pairs of endometrial cancer tissues and normal endometrial tissue specimens were collected,and the expression levels of CNTN-1 protein in them were determined by Western Blotting.The clinical data of all patients,including information on age,menstrual status,surgical method and pathological data,were also collected,and the test results were compiled to analyze the relationship between the differences in expression levels and the characteristics of the clinicopathological data.Result1.Western Blotting results showed that the expression level of CNTN-1 protein was higher in endometrial cancer lesions than in normal endometrium.2.Immunohistochemistry showed that CNTN-1 protein expression was significantly higher in endometrial cancer tissues than in normal tissues.3.There was no statistical difference in the intensity of positive CNTN-1 protein expression in different age subgroups,in subgroups of menstrual status,in groups grouped by FIGO clinical stage,degree of tumor differentiation,and Ki-67 expression level(P>0.05).Conclusion1.The expression of CNTN-1 protein was significantly increased in endometrial adenocarcinoma lesion tissues.It is suggested that CNTN-1 protein may be a protein with a key role in the pathogenesis,infiltration,and metastasis of endometrial adenocarcinoma.2.The abnormal expression of CNTN-1 protein was also not correlated with age,menstrual status,stage of endometrial cancer,Ki-67 expression level,and pathological differentiation grade. |
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