Study On The Role Of USP5 In Collagen Synthesis In Inflammation And Diabetes Model

Objective: To elucidate the role of USP5 on collagen synthesis in inflammatory and diabetic models,and to provide new molecular targets and theoretical basis for the treatment of diabetic renal fibrosis.Methods:A mouse inflammation model was constructed using LPS to detect changes in the expression of USP5,inflammation and collagen synthesis-related factors in kidney tissues.A model of inflammation in HK-2 cells was constructed using LPS to detect changes in the expression of USP5,inflammation and collagen synthesis-related factors.Suppression of USP5 expression by RNA interference technology and detection of expression changes of USP5 and inflammation-related factors.H&E staining,Masson staining and immunohistochemical staining were used to observe the histopathological changes and collagen expression in the mice,and USP5 and collagen synthesis-related factors were detected by immunofluorescence and Western blot.The expression changes of USP5 and collagen synthesis-related factors were detected by immunofluorescence and Western blot.A hyperlipidemic model of HK-2 cells was constructed using different concentrations of PA,and the expression of USP5 was inhibited by RNA interference technology to detect changes in the expression of related genes before and after USP5 expression inhibition.Results:In the mouse inflammation model: the expression levels of USP5,inflammation and collagen synthesis-related factors were elevated in the LPS group compared to the CON group;in the HK-2 cell inflammation model: the protein and m RNA expression levels of USP5,inflammation and collagen synthesis-related factors were elevated in the LPS group compared to the CON group;the protein and m RNA expression levels of USP5 and inflammation-related factors were downregulated in the si USP5 group compared to the si NC group.In the mouse diabetic model: compared with the CON group,the H&E staining results of the DM group showed glomerular wrinkling,tubular atrophy,tubular dilatation,vacuolar deformation and detachment of tubular epithelial cells,and infiltration of interstitial inflammatory cells;the Masson staining results of the DM group showed significant collagen deposition;the immunohistochemical results of the DM group showed increased expression of COLI and COLIII;the protein expression levels of collagen synthesis-related factors were increased in the DM group.In the cellular hyperlipidemia model: the protein and m RNA expression levels of USP5 and collagen synthesis-related factors were elevated in the PA group compared to the CON group.The protein and m RNA expression levels of USP5 and collagen synthesis-related factors were down-regulated in the si USP5 group compared to the si NC group.Conclusion : USP5 was highly expressed in the diabetic and inflammatory models.Inhibition of USP5 expression resulted in a significant decrease in the expression levels of inflammation and collagen synthesis-related factors,suggesting that USP5 promotes collagen synthesis in the inflammation and diabetes models.